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Correlation between Mutations in
<i>liaFSR</i>
of Enterococcus faecium and MIC of Daptomycin: Revisiting Daptomycin Breakpoints
(2012)
MUNITA SEPULVEDA, JOSE MANUEL
;
Diana Panesso
;
Lorena Diaz
;
Truc T. Tran
;
Jinnethe Reyes
Mutations in liaFSR, a three-component regulatory system controlling cell-envelope stress response, were recently linked with the emergence of daptomycin (DAP) resistance in enterococci. Our previous work showed that a liaF mutation increased the DAP MIC of a vancomycin-resistant Enterococcus faecalis strain from 1 to 3 μg/ml (the DAP breakpoint is 4 μg/ml), suggesting that mutations in the liaFSR system could be a pivotal initial event in the development of DAP resistance. With the hypothesis that clinical enterococcal isolates with DAP MICs between 3 and 4 μg/ml might harbor mutations in liaFSR, we studied 38 Enterococcus faecium bloodstream isolates, of which 8 had DAP MICs between 3 and 4 μg/ml by Etest in Mueller-Hinton agar. Interestingly, 6 of these 8 isolates had predicted amino acid changes in the LiaFSR system. Moreover, we previously showed that among 6 DAP-resistant E. faecium isolates (MICs of >4 μg/ml), 5 had mutations in liaFSR. In contrast, none of 16 E. faecium isolates with a DAP MIC of ≤2 μg/ml harbored mutations in this system (P<0.0001). All but one isolate with liaFSR changes exhibited DAP MICs of ≥16 μg/ml by Etest using brain heart infusion agar (BHIA), a medium that better supports enterococcal growth. Our findings provide a strong association between DAP MICs within the upper susceptibility range and mutations in the liaFSR system. Concomitant susceptibility testing on BHIA may be useful for identifying these E. faecium first-step mutants. Our results also suggest that the current DAP breakpoint for E. faecium may need to be reevaluated
14Scopus© Citations 104
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The amniotic fluid as a source of mesenchymal stem cells with lung‐specific characteristics
(2017)
Flore Lesage
;
Silvia Zia
;
JIMENEZ GREZ, JULIO ALBERTO
;
Jan Deprest
;
Jaan Toelen
<jats:p>What is already known about this topic? <jats:list list-type="bullet"> <jats:list-item><jats:p>Fetal tissues are interesting sources of stem cells for autologous therapy in a neonatal context.</jats:p></jats:list-item> <jats:list-item><jats:p>AF‐MSC are a heterogeneous stem cell population of which the exact origin remains elusive.</jats:p></jats:list-item> </jats:list></jats:p><jats:p>What does this study add? <jats:list list-type="bullet"> <jats:list-item><jats:p>This study identifies AF‐MSC with lung‐specific characteristics.</jats:p></jats:list-item> <jats:list-item><jats:p>This study provides a reliable culture method that supports the expansion of lung‐specific AF‐MSC.</jats:p></jats:list-item> </jats:list></jats:p>
8Scopus© Citations 6
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The Antidiabetic Effect of MSCs Is Not Impaired by Insulin Prophylaxis and Is Not Improved by a Second Dose of Cells
(2011)
EZQUER, EDUARDO FERNANDO
;
EZQUER, EDUARDO MARCELO
;
Valeska Simon
;
CONGET MOLINA, PAULETTE ANDREA
;
Kathrin Maedler
10 1Scopus© Citations 28
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Preconditioning of adipose tissue-derived mesenchymal stem cells with deferoxamine increases the production of pro-angiogenic, neuroprotective and anti-inflammatory factors: Potential application in the treatment of diabetic neuropathy
(2017)
Carolina Oses
;
OLIVARES, MARIA BELEN
;
EZQUER, EDUARDO MARCELO
;
Cristian Acosta
;
BOSCH PÉREZ, PAUL JESÚS
6Scopus© Citations 126
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Exosomes on the border of species and kingdom intercommunication
(2019)
SCHUH, CHRISTINA
;
Jimena Cuenca
;
Francisca Alcayaga-Miranda
;
Maroun Khoury
1Scopus© Citations 48 4
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A Tetrameric Peptide Derived from Bovine Lactoferricin Exhibits Specific Cytotoxic Effects against Oral Squamous-Cell Carcinoma Cell Lines
(2015)
Víctor A. Solarte
;
Jaiver E. Rosas
;
Zuly J. Rivera
;
Martha L. Arango-Rodríguez
;
Javier E. García
<jats:p>Several short linear peptides derived from cyclic bovine lactoferricin were synthesized and tested for their cytotoxic effect against the oral cavity squamous-cell carcinoma (OSCC) cell lines CAL27 and SCC15. As a control, an immortalized and nontumorigenic cell line, Het-1A, was used. Linear peptides based on the RRWQWR core sequence showed a moderate cytotoxic effect and specificity towards tumorigenic cells. A tetrameric peptide, LfcinB(20–25)<jats:sub>4</jats:sub>, containing the RRWQWR motif, exhibited greater cytotoxic activity (>90%) in both OSCC cell lines compared to the linear lactoferricin peptide or the lactoferrin protein. Additionally, this tetrameric peptide showed the highest specificity towards tumorigenic cells among the tested peptides. Interestingly, this effect was very fast, with cell shrinkage, severe damage to cell membrane permeability, and lysis within one hour of treatment. Our results are consistent with a necrotic effect rather than an apoptotic one and suggest that this tetrameric peptide could be considered as a new candidate for the therapeutic treatment of OSCC.</jats:p>
Scopus© Citations 33
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The role of bone marrow mesenchymal stromal cell derivatives in skin wound healing in diabetic mice
(2017)
Tomas de Mayo
;
CONGET MOLINA, PAULETTE ANDREA
;
Silvia Becerra-Bayona
;
Claudia L. Sossa
;
Virgilio Galvis
Scopus© Citations 65 6
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Pro-Inflammatory Chemokines and Cytokines Dominate the Blister Fluid Molecular Signature in Patients with Epidermolysis Bullosa and Affect Leukocyte and Stem Cell Migration
(2017)
Vitali Alexeev
;
Julio Cesar Salas-Alanis
;
FRANCIS PALISSON ETCHARREN
;
Lila Mukhtarzada
;
Giulio Fortuna
11Scopus© Citations 40
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Comparative study of the neural differentiation capacity of mesenchymal stromal cells from different tissue sources: An approach for their use in neural regeneration therapies
(2019)
Daniela N. Urrutia
;
Pablo Caviedes
;
Rodrigo Mardones
;
José J. Minguell
;
ANA MARIA FERNANDA VEGA LETTER
27Scopus© Citations 103

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