SCHUH, CHRISTINA

Preferred name

SCHUH, CHRISTINA

Main Affiliation

Facultad de Medicina Clínica Alemana Universidad del Desarrollo

cschuh@udd.cl

ORCID

0000-0001-9475-4513

Scopus Author ID

55897063400

Goggle Scholar

https://scholar.google.com/citations?user=61QqHmwAAAAJ&hl=es&oi=ao

Now showing 1 - 10 of 20

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Dataset - Biofilm formation on collagen substrates modulates Streptococcus mutans bacterial extracellular nanovesicle production and cargo
(Figshare (United Kingdom), 2025)
JAVIERA CARRASCO
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SCHUH, CHRISTINA
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Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging
(2024)
Simon Álvarez
;
Jose Morales
;
Paola Tiozzo-Lyon
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Pablo Berrios
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Valentina Barraza
<jats:p>Our study aimed to develop a novel <jats:italic>in vitro</jats:italic> microfabricated biomimetic dentin surface that simulates the complex surface microarchitecture of exposed dentin, as well as age-derived glycation of teeth, for the growth of polymicrobial oral biofilms.</jats:p>
7
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Royal jelly derived extracellular vesicles modulate microglial nanomechanics and inflammatory responses
(Royal Society of Chemistry (RSC), 2025)
Gabriela Zavala
;
Pablo Berríos
;
Felipe Sandoval
;
Graciela Bravo
;
Nelson P. Barrera
Royal jelly-derived extracellular vesicles counteract inflammation-induced mechanobiological alterations in microglia, restoring their mechanical properties and offering a novel interkingdom approach to modulate neuroinflammation.
Scopus© Citations 1 3
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NK-Extrusomes: Artificial Cell-Derived Vesicles (ACDV), An Alternative Nanodrug Delivery System for Lung Cancer
(2025)
CARRASCO, JAVIERA
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ORLANDO JOSUE RAMIREZ PRADA
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GABRIELA ZAVALA VIVAR
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RAFAEL ALEXANDER CONTRERAS LÓPEZ
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FELIPE I. SANDOVAL
5
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Artificial cell-derived vesicles by extrusion, a novel docetaxel drug delivery system for lung cancer
(Elsevier BV, 2025-04)
Javiera Carrasco-Rojas
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Gabriela Zavala
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Rafael Contreras-Lopez
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OLIVARES, MARIA BELEN
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Miriam Aarsund
Scopus© Citations 1 7
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Biofilm formation on collagen substrates modulates <i>Streptococcus mutans</i> bacterial extracellular nanovesicle production and cargo
(Royal Society of Chemistry (RSC), 2025)
Camila Leiva-Sabadini
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Pablo Berríos
;
Paula Saavedra
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Javiera Carrasco-Rojas
;
José Vicente González-Aramundiz
Streptococcus mutans is the major microbial etiological agent of dental caries and can adhere to surfaces such as type-I collagen, which is present in dentin and periodontal tissues. Recent studies have characterized planktonic S. mutans bacterial extracellular vesicles (bEVs) at the nanoscale range and demonstrated environmental-induced changes due to sugar presence or pH alterations. However, to date, no studies have explored whether surface-derived changes can modulate bEV production in the context of oral biofilm formation in the elderly. Therefore, this work aimed to determine the role of biofilm formation and collagen glycation on the nanoscale morphology and proteomic composition of S. mutans bEVs. For this, bEVs from S. mutans biofilms on native and glycated collagen surfaces were isolated, characterized, and compared to bEVs from planktonic cells. Nanoparticle tracking analysis (NTA), atomic force microscopy (AFM), and electron microscopy confirmed bEV production and showed that bEVs from biofilms are smaller in size and less abundant than those from planktonic cells. Furthermore, proteome analysis revealed that S. mutans biofilm formation on native and glycated collagen led to the enrichment of several key virulence proteins. Also, a shift towards proteins involved in metabolic processes was found in bEVs following biofilm formation on collagen surfaces, whereas glucan metabolism proteins were overexpressed in vesicles from the planktonic state. These results demonstrate that biofilm formation, as well as the glycation of collagen associated with aging and hyperglycaemia, can modulate bEV characteristics and cargo and could play a central role in S. mutans virulence and the development of diseases such as dental caries and periodontal disease.
2Scopus© Citations 4
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Modulation of the biophysical and biochemical properties of collagen by glycation for tissue engineering applications
(2023)
Mina Vaez
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Meisam Asgari
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Liisa Hirvonen
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Gorkem Bakir
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Emilie Khattignavong
5 1Scopus© Citations 21
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Streptococcus mutans Extracellular Vesicles Regulate Early Streptococcus sanguinis Nanoadhesion
(SAGE Publications, 2026-02-24)
C. Leiva-Sabadini
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C.M. Lévesque
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L. Bozec
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SCHUH, CHRISTINA
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S. Aguayo
<jats:p> Dental caries remains a globally prevalent disease, driven by microbial dysbiosis and competitive ecological shifts within the oral biofilm. A key interaction in this context is the antagonism between <jats:italic toggle="yes">Streptococcus mutans</jats:italic> and <jats:italic toggle="yes">Streptococcus sanguinis</jats:italic> , two early colonizers of the dentin surfaces. While bacterial extracellular vesicles (bEVs) have emerged as important mediators of microbial communication, their role in interspecies adhesion and early biofilm dynamics remains poorly understood. This study explored how <jats:italic toggle="yes">S. mutans</jats:italic> –derived bEVs, isolated from planktonic cultures and biofilms grown on native or glycated type I collagen substrates, modulate <jats:italic toggle="yes">S. sanguinis</jats:italic> nanoadhesion and initial biofilm formation. Bacterial EVs were characterized using nanoparticle tracking analysis and transmission electron microscopy, and their functional effects were assessed via atomic force microscopy–based single-cell force spectroscopy. This approach enabled direct quantification of bacterial adhesion forces and single-molecule unbinding events under different vesicle-exposure conditions. Our results demonstrated that planktonic-derived bEVs enhanced <jats:italic toggle="yes">S. sanguinis</jats:italic> adhesion at 5-s contact times, while bEVs from collagen-bound <jats:italic toggle="yes">S. mutans</jats:italic> biofilms, particularly those grown on glycated matrices, consistently reduced adhesion forces, rupture lengths, and unbinding events. Optical coherence tomography imaging confirmed that these nanoscale effects translated into altered early biofilm architecture, with planktonic bEVs promoting thicker, denser biofilms and biofilm-derived bEVs leading to sparser colonization. Our findings suggest that <jats:italic toggle="yes">S. mutans</jats:italic> bEVs exhibit a context-dependent modulatory effect on <jats:italic toggle="yes">S. sanguinis</jats:italic> , enhancing adhesion under planktonic conditions and suppressing it upon biofilm establishment. This biphasic behavior may represent a strategic mechanism for niche domination during caries initiation. Moreover, collagen glycation—mimicking aged or hyperglycemic dentin—further influenced bEV function, underscoring the importance of the host matrix state in microbial interactions. Overall, this study highlights a previously unrecognized role for bEVs in shaping early oral dysbiosis at the single-cell level. </jats:p>
Scopus© Citations 1 1
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Glycation Promotes Oral Biofilm Formation On Microfabricated Bioengineered Dentin Substrates
(2024)
Alvarez, S
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Morales, J.
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Teuschl-Woller, A.
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SCHUH, CHRISTINA
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AGUAYO, S.
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Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses
(2023)
Simón Álvarez
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Pamina Contreras-Kallens
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Sebastian Aguayo
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Ramírez Prada, Orlando
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Catalina Vallejos
1Scopus© Citations 17 4

SCHUH, CHRISTINA

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