Multispecies emergence of dual blaKPC/NDM carbapenemase-producing Enterobacterales recovered from invasive infections in Chile

Journal
Antimicrobial Agents and Chemotherapy
ISSN
0066-4804
Date Issued
2025-01-31
Author(s)
Camila Solar
Jose R. W. Martínez
Valeria Quiroz
Ana M. González
Roberto Riquelme-Neira
Juan A. Ugalde
Anne Peters
Oscar Ortega-Recalde
Patricia García
Francois Lebreton
Type
journal-article
DOI
URL
Abstract
Carbapenemase-producing carbapenem-resistant Enterobacterales (CP-CRE) represent a significant global threat. The emergence of dual CP-CRE is particularly alarming, as they can potentially compromise the efficacy of newer antibiotics, further decreasing therapeutic alternatives. Herein, we report the emergence of multiple species of CP-CRE recovered from invasive infections in Chile that simultaneously harbor and provide an in-depth genomic characterization of these worrisome pathogens. We collected carbapenem-resistant Enterobacterales (CRE) isolates from invasive infections over a 4-year period, across 11 healthcare centers in Chile. Bacterial species and the presence of carbapenemase genes were confirmed using MALDI-TOF and PCR assays, respectively. Antimicrobial susceptibility testing was conducted through disk diffusion and broth microdilution methods. Dual CP-CRE isolates were subjected to short- and long-read whole genome sequencing to perform a detailed genomic characterization of the isolates and of the mobile genetic elements harboring the enzymes. From a total of 1,335 CRE isolates, we observed an increase in the prevalence of CP-CRE, from 11% in 2019 to 38% in 2022. A total of 11 dual CP-CRE isolates were recovered, all of them harboring
Project(s)
Dataset(s)
Subjects
anti-bacterial agents

; 

bacterial proteins

; 

beta-lactamases

; 

carbapenem-resistant enterobacteriaceae

; 

carbapenems

; 

chile

; 

enterobacteriaceae infections

; 

humans

; 

klebsiella pneumoniae

; 

microbial sensitivity tests

; 

plasmids

; 

whole genome sequencing

; 

antiinfective agent

; 

bacterial protein

; 

beta lactamase

; 

carbapenem derivative

; 

carbapenemase

; 

carbapenem-resistant enterobacteriaceae

; 

chile

; 

drug effect

; 

drug therapy

; 

enterobacteriaceae infection

; 

epidemiology

; 

genetics

; 

human

; 

isolation and purification

; 

klebsiella pneumoniae

; 

microbial sensitivity test

; 

microbiology

; 

plasmid

; 

whole genome sequencing