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Project Title
Analysis of antimicrobial and pro-regenerative properties of exosomes derived from Apis mellifera Royel Jelly
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Internal ID
11180406
Principal Investigator
Start Date
2018

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Exosome-like vesicles in Apis mellifera bee pollen, honey and royal jelly contribute to their antibacterial and pro-regenerative activity

2019 , Christina M. A. P. Schuh , Sebastian Aguayo , Gabriela Zavala , Maroun Khoury

Microvesicles have become key players in cellular communication. Since glandular secretions present a rich source of active exosomes, we hypothesized that exosome-like vesicles are present in Apis mellifera hypopharyngeal gland secretomal products (honey, royal jelly and bee pollen), and participate in their known antibacterial and pro-regenerative effects. We developed an isolation protocol based on serial- and ultracentrifugation steps and demonstrated the presence of protein-containing exosome-like vesicles in all three bee-derived products. Assessing their antibacterial properties, we found that exosome-like vesicles had bacteriostatic, bactericidal and biofilm-inhibiting effects on Staphylococcus aureus. Furthermore, we could demonstrate that mesenchymal stem cells (MSCs) internalize bee-derived exosome-like vesicles and that these vesicles influence their migration potential. In an in vitro wound healing assay, honey and royal jelly exosome-like vesicles increased migration of human MSC, demonstrating their interkingdom activity. Summarizing, we have discovered exosome-like vesicles as a new, active compound in bee pollen, honey and royal jelly.

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Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles

2020 , Orlando J. Ramírez , Simón Alvarez , Pamina Contreras-Kallens , Nelson P. Barrera , Sebastian Aguayo , Christina M. A. P. Schuh

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Antibacterial Effect of Honey-Derived Exosomes Containing Antimicrobial Peptides Against Oral Streptococci

2021 , Camila Leiva-Sabadini , Simon Alvarez , Nelson P Barrera , Christina MAP Schuh , Sebastian Aguayo

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Exosomes on the border of species and kingdom intercommunication

2019 , SCHUH, CHRISTINA , Jimena Cuenca , Francisca Alcayaga-Miranda , Maroun Khoury

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Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses

2023 , Simón Álvarez , Pamina Contreras-Kallens , Sebastian Aguayo , Ramírez Prada, Orlando , Catalina Vallejos , Jorge Ruiz , Eva Carrasco-Gallardo , Stefanie Troncoso-Vera , Bernardo Morales , SCHUH, CHRISTINA

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Complex Interaction between Resident Microbiota and Misfolded Proteins: Role in Neuroinflammation and Neurodegeneration

2020 , Juliana González-Sanmiguel , Christina M. A. P. Schuh , Carola Muñoz-Montesino , Pamina Contreras-Kallens , Luis G. Aguayo , Sebastian Aguayo

Neurodegenerative diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and Creutzfeldt–Jakob disease (CJD) are brain conditions affecting millions of people worldwide. These diseases are associated with the presence of amyloid-β (Aβ), alpha synuclein (α-Syn) and prion protein (PrP) depositions in the brain, respectively, which lead to synaptic disconnection and subsequent progressive neuronal death. Although considerable progress has been made in elucidating the pathogenesis of these diseases, the specific mechanisms of their origins remain largely unknown. A body of research suggests a potential association between host microbiota, neuroinflammation and dementia, either directly due to bacterial brain invasion because of barrier leakage and production of toxins and inflammation, or indirectly by modulating the immune response. In the present review, we focus on the emerging topics of neuroinflammation and the association between components of the human microbiota and the deposition of Aβ, α-Syn and PrP in the brain. Special focus is given to gut and oral bacteria and biofilms and to the potential mechanisms associating microbiome dysbiosis and toxin production with neurodegeneration. The roles of neuroinflammation, protein misfolding and cellular mediators in membrane damage and increased permeability are also discussed.

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Potential Novel Strategies for the Treatment of Dental Pulp-Derived Pain: Pharmacological Approaches and Beyond

2019 , SCHUH, CHRISTINA , Bruna Benso , Sebastian Aguayo

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