Exosome-like vesicles in Apis mellifera bee pollen, honey and royal jelly contribute to their antibacterial and pro-regenerative activity
Journal
Journal of Experimental Biology
ISSN
1477-9145
0022-0949
Date Issued
2019
Author(s)
Christina M. A. P. Schuh
Sebastian Aguayo
Gabriela Zavala
Maroun Khoury
Type
Resource Types::text::journal::journal article
Abstract
<jats:p>Microvesicles have become key players in cellular communication. Since glandular secretions present a rich source of active exosomes, we hypothesized that exosome-like vesicles are present in Apis mellifera hypopharyngeal gland secretomal products (honey, royal jelly and bee pollen), and participate in their known antibacterial and pro-regenerative effects. We developed an isolation protocol based on serial- and ultracentrifugation steps and demonstrated the presence of protein-containing exosome-like vesicles in all three bee-derived products. Assessing their antibacterial properties, we found that exosome-like vesicles had bacteriostatic, bactericidal and biofilm-inhibiting effects on Staphylococcus aureus. Furthermore, we could demonstrate that mesenchymal stem cells (MSCs) internalize bee-derived exosome-like vesicles and that these vesicles influence their migration potential. In an in vitro wound healing assay, honey and royal jelly exosome-like vesicles increased migration of human MSC, demonstrating their interkingdom activity. Summarizing, we have discovered exosome-like vesicles as a new, active compound in bee pollen, honey and royal jelly.</jats:p>
Cite this document
Schuh, C. M. A. P., Aguayo, S., Zavala, G., & Khoury, M. (2019). Exosome-like vesicles in Apis mellifera bee pollen, honey and royal jelly contribute to their antibacterial and pro-regenerative activity. Journal of Experimental Biology, jeb.208702. https://doi.org/10.1242/jeb.208702
Subjects
microvesicle
;
apis mellifera
;
bactericide
;
intercellular communication
;
inter-kingdom communication
;
animals
;
anti-bacterial agents
;
bees
;
cell movement
;
endocytosis
;
exosomes
;
fatty acids
;
honey
;
humans
;
pollen
;
regeneration
;
antiinfective agent
;
fatty acid
;
royal jelly
;
animal
;
bee
;
cell motion
;
chemistry
;
drug effect
;
endocytosis
;
exosome
;
honey
;
human
;
metabolism
;
pollen
;
regeneration
;
ultrastructure