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    Treatment of Patellofemoral Chondral Lesions Using Microfractures Associated with a Chitosan Scaffold: Mid-Term Clinical and Radiological Results
    (2021)
    CALVO RODRIGUEZ, RAFAEL  
    ;
    Jose Bravo
    ;
    FIGUEROA POBLETE, DAVID HUMBERTO  
    ;
    FIGUEROA BERRIOS, FRANCISCO JAVIER  
    ;
    Martin Contreras
    <jats:sec><jats:title>Objective</jats:title><jats:p> To assess the clinical and radiological results of patellofemoral osteochondral lesions treated with microfractures associated with a chitosan scaffold. </jats:p></jats:sec><jats:sec><jats:title>Design</jats:title><jats:p> A retrospective observational analytical study was performed. Fifteen patients with full-thickness patellofemoral osteochondral lesions were included. Quantity and quality of the reparation cartilage was assessed with the MOCART 2.0 score on a postoperative magnetic resonance imaging (MRI), and clinical outcomes were evaluated with pre- and postoperative Kujala score tests. Shapiro-Wilk test for normality was applied as well as Wilcoxon’s signed rank test and Kruskal-Wallis H test for clinical scores within subjects and patella versus trochlea subgroups comparisons. Analysis of variance test was used for imaging subgroups comparison, with P &lt; 0.05 defined as statistical significance. </jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p> Mean follow-up was 33.36 months (range 24-60 months). Postoperative Kujala scores improved an average of 19 points compared with the preoperative state (SE = 17.6; P &lt; 0.001). No statistical difference was found through the clinical location assessment ( P = 0.756), as well as the cartilage imaging assessment ( P = 0.756). The mean MOCART 2.0 scale was 67.67 (range 50-85). </jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p> Treating full-thickness patellofemoral osteochondral lesions with microfractures associated with a chitosan scaffold proved to be effective regarding defect filling and symptomatic improvement. </jats:p></jats:sec>
    Scopus© Citations 6  1
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    Assessment of Cell Viability of Fresh Osteochondral Allografts in N-Acetylcysteine-Enriched Medium
    (2020)
    CALVO RODRIGUEZ, RAFAEL  
    ;
    Maximiliano Espinosa
    ;
    David Figueroa
    ;
    Luz María Pozo
    ;
    CONGET MOLINA, PAULETTE ANDREA  
    <jats:sec><jats:title>Objective</jats:title><jats:p> The purpose of this study was to evaluate the effect of N-acetylcysteine (NAC)-enriched storage medium on fresh osteochondral viability at 4°C. Our hypothesis was that the cell viability of chondrocytes obtained from human osteochondral tissue and stored at 4°C significantly improves in the presence of NAC. </jats:p></jats:sec><jats:sec><jats:title>Design</jats:title><jats:p> Controlled laboratory study. For this study, 8 samples of femoral condyle osteochondral tissue were obtained from patients undergoing total knee replacement. The samples were stored at either 4°C in phosphate-buffered saline (PBS) or at 3 different concentrations of NAC (NAC 1, 2, and 5 mM). Cell viability was analyzed at time 0 and 4 weeks by flow cytometry. The results of cell viability (median) were analyzed statistically using analysis of variance and Tukey’s post hoc test. P values &lt;0.05 were considered statistically significant. </jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p> The viability at time 0 was 95.5% ± 3.7%. At 4 weeks, the cell viability was 56.8% ± 20.1% in the control group (PBS), 83.8% ± 11.9% in the group stored with NAC 1 mM, 73.4% ± 13.6% in the group stored with NAC 2 mM, and 66.4% ± 27.7% in the group stored with NAC 5 mM. A statistically significant difference from the baseline viability (time 0) was observed in the PBS control group ( P = 0.0018) but not in the other groups. A statistically significant difference was observed in the NAC 1 mM group compared with the PBS group ( P = 0.0255). </jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p> The use of NAC at 1 mM concentration improves cell viability after 4 weeks of storage in chondrocytes obtained from human osteochondral tissue. </jats:p></jats:sec>
      1  1Scopus© Citations 11
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    Specific, Sensitive, and Stable Reporting of Human Mesenchymal Stromal Cell Chondrogenesis
    (2019)
    Rodolfo E. De la Vega
    ;
    ALFREDO MAXIMILIANO SCHEU GONCALVES
    ;
    Lennart A. Brown
    ;
    Christopher H. Evans
    ;
    Elisabeth Ferreira
      1Scopus© Citations 7