Assessment of Cell Viability of Fresh Osteochondral Allografts in N-Acetylcysteine-Enriched Medium
Journal
CARTILAGE
ISSN
1947-6035
1947-6043
Date Issued
2020
Author(s)
Maximiliano Espinosa
David Figueroa
Luz María Pozo
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:sec><jats:title>Objective</jats:title><jats:p> The purpose of this study was to evaluate the effect of N-acetylcysteine (NAC)-enriched storage medium on fresh osteochondral viability at 4°C. Our hypothesis was that the cell viability of chondrocytes obtained from human osteochondral tissue and stored at 4°C significantly improves in the presence of NAC. </jats:p></jats:sec><jats:sec><jats:title>Design</jats:title><jats:p> Controlled laboratory study. For this study, 8 samples of femoral condyle osteochondral tissue were obtained from patients undergoing total knee replacement. The samples were stored at either 4°C in phosphate-buffered saline (PBS) or at 3 different concentrations of NAC (NAC 1, 2, and 5 mM). Cell viability was analyzed at time 0 and 4 weeks by flow cytometry. The results of cell viability (median) were analyzed statistically using analysis of variance and Tukey’s post hoc test. P values <0.05 were considered statistically significant. </jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p> The viability at time 0 was 95.5% ± 3.7%. At 4 weeks, the cell viability was 56.8% ± 20.1% in the control group (PBS), 83.8% ± 11.9% in the group stored with NAC 1 mM, 73.4% ± 13.6% in the group stored with NAC 2 mM, and 66.4% ± 27.7% in the group stored with NAC 5 mM. A statistically significant difference from the baseline viability (time 0) was observed in the PBS control group ( P = 0.0018) but not in the other groups. A statistically significant difference was observed in the NAC 1 mM group compared with the PBS group ( P = 0.0255). </jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p> The use of NAC at 1 mM concentration improves cell viability after 4 weeks of storage in chondrocytes obtained from human osteochondral tissue. </jats:p></jats:sec>
Cite this document
Calvo, R., Espinosa, M., Figueroa, D., Pozo, L. M., & Conget, P. (2020). Assessment of cell viability of fresh osteochondral allografts in n -acetylcysteine-enriched medium. CARTILAGE, 11(1), 117-121. https://doi.org/10.1177/1947603518786547
Subjects
osteochondral allografts
;
chondrocyte viability
;
storage medium
;
tissue banking
;
acetylcysteine
;
allografts
;
cell survival
;
chondrocytes
;
culture media
;
femur
;
humans
;
tissue preservation
;
acetylcysteine
;
acetylcysteine
;
article
;
articular cartilage
;
bone allograft
;
cell viability
;
chondrocyte
;
clinical article
;
controlled study
;
female
;
femoral condyle
;
flow cytometry
;
human
;
human cell
;
human tissue
;
knee replacement
;
male
;
osteochondral allograft
;
post hoc analysis
;
quantitative analysis
;
subchondral bone
;
total knee arthroplasty
;
allograft
;
cell survival
;
culture medium
;
cytology
;
drug effect
;
femur
;
procedures
;
tissue preservation