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Author: search.filters.author.Andrew FG QuestSubject: search.filters.subject.metastasis

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    Caveolin-1-dependent tenascin C inclusion in extracellular vesicles is required to promote breast cancer cell malignancy
    (2023)
    America Campos
    ;
    Renato Burgos-Ravanal
    ;
    Lorena Lobos-González
    ;
    Ricardo Huilcamán
    ;
    María Fernanda González
    <jats:p> Background: Elevated expression of CAV1 in breast cancer increases tumor progression. Extracellular vesicles (EVs) from CAV1-expressing MDA-MB-231 breast cancer cells contain Tenascin C (TNC), but the relevance of TNC remained to be defined. Methods: EVs were characterized by nanotracking analysis, microscopy and western blotting. The uptake of EVs by cells was studied using flow cytometry. The effects of EVs on breast cancer cells were tested in migration, invasion, colony formation and in vivo assays. Results: EVs were taken up by cells; however, only those containing TNC promoted invasiveness. In vivo, EVs lacking TNC ceased to promote tumor growth. Conclusion: CAV1 and TNC contained in breast cancer cell-derived EVs were identified as proteins that favor progression of breast cancer. </jats:p>
    Scopus© Citations 7  1
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    Gold nanoparticles as tracking devices to shed light on the role of caveolin-1 in early stages of melanoma metastasis
    (2018)
    Simón Guerrero
    ;
    Victor Manuel Díaz-García
    ;
    Pamela Contreras-Orellana
    ;
    Pablo Lara
    ;
    Sujey Palma
    <jats:p> Aim: To track early events during lung metastasis, we labeled cells expressing (B16F10<jats:sub>CAV1</jats:sub>) or lacking CAV1 (B16F10<jats:sub>mock</jats:sub>) with gold nanoparticles conjugated to the peptide TAT (AuNPs-PEG-TAT). Methods: B16F10 expressing or lacking CAV1 were labeled with AuNPs-PEG-TAT. The physicochemical properties and cytotoxicity of these nanoparticles, as well as their effects on migration and invasiveness of B16F10 cells in vitro were evaluated. Ex vivo lung distribution of the labeled cells after tail vein injection into C57BL/6 mice was examined. Results: AuNPs-PEG-TAT did not affect B16F10 viability, migration and invasiveness. The metastatic and tumorigenic capability of the labeled B16F10 was also not modified in comparison to unlabeled B16F10 cells. CAV1 expression favored the retention of B16F10 cells in the lungs of mice 2 h post injection, suggesting CAV1 promoted adherence to endothelial cells and transendothelial migration. Conclusions: We developed a protocol to label B16F10 cells with AuNPs-PEG-TAT that permits subsequent tracking of cells in mice. CAV1 overexpression was found to increase retention and transendothelial migration of B16F10 cells in the lung. </jats:p>
      1Scopus© Citations 9  1