Gold nanoparticles as tracking devices to shed light on the role of caveolin-1 in early stages of melanoma metastasis
Journal
Nanomedicine
ISSN
1743-5889
1748-6963
Date Issued
2018
Author(s)
Simón Guerrero
Victor Manuel Díaz-García
Pamela Contreras-Orellana
Pablo Lara
Sujey Palma
Fanny Guzman
Areli Cárdenas
Ximena Rojas-Silva
Luis Muñoz
Lisette Leyton
Marcelo J Kogan
Andrew FG Quest
Type
Resource Types::text::journal::journal article
Abstract
<jats:p> Aim: To track early events during lung metastasis, we labeled cells expressing (B16F10<jats:sub>CAV1</jats:sub>) or lacking CAV1 (B16F10<jats:sub>mock</jats:sub>) with gold nanoparticles conjugated to the peptide TAT (AuNPs-PEG-TAT). Methods: B16F10 expressing or lacking CAV1 were labeled with AuNPs-PEG-TAT. The physicochemical properties and cytotoxicity of these nanoparticles, as well as their effects on migration and invasiveness of B16F10 cells in vitro were evaluated. Ex vivo lung distribution of the labeled cells after tail vein injection into C57BL/6 mice was examined. Results: AuNPs-PEG-TAT did not affect B16F10 viability, migration and invasiveness. The metastatic and tumorigenic capability of the labeled B16F10 was also not modified in comparison to unlabeled B16F10 cells. CAV1 expression favored the retention of B16F10 cells in the lungs of mice 2 h post injection, suggesting CAV1 promoted adherence to endothelial cells and transendothelial migration. Conclusions: We developed a protocol to label B16F10 cells with AuNPs-PEG-TAT that permits subsequent tracking of cells in mice. CAV1 overexpression was found to increase retention and transendothelial migration of B16F10 cells in the lung. </jats:p>
Subjects
cell labeling
;
cell tracking
;
gold nanoparticles
;
metastasis
;
theranostics