Caveolin-1-dependent tenascin C inclusion in extracellular vesicles is required to promote breast cancer cell malignancy
Journal
Nanomedicine
ISSN
1743-5889
1748-6963
Date Issued
2023
Author(s)
America Campos
Renato Burgos-Ravanal
Lorena Lobos-González
Ricardo Huilcamán
María Fernanda González
Jorge Díaz
Albano Cáceres Verschae
Juan Pablo Acevedo
Macarena Carrasco
Francisca Sepúlveda
Emanuel Jeldes
Manuel Varas-Godoy
Lisette Leyton
Andrew FG Quest
Type
Resource Types::text::journal::journal article
Abstract
<jats:p> Background: Elevated expression of CAV1 in breast cancer increases tumor progression. Extracellular vesicles (EVs) from CAV1-expressing MDA-MB-231 breast cancer cells contain Tenascin C (TNC), but the relevance of TNC remained to be defined. Methods: EVs were characterized by nanotracking analysis, microscopy and western blotting. The uptake of EVs by cells was studied using flow cytometry. The effects of EVs on breast cancer cells were tested in migration, invasion, colony formation and in vivo assays. Results: EVs were taken up by cells; however, only those containing TNC promoted invasiveness. In vivo, EVs lacking TNC ceased to promote tumor growth. Conclusion: CAV1 and TNC contained in breast cancer cell-derived EVs were identified as proteins that favor progression of breast cancer. </jats:p>
Cite this document
Campos, A., Burgos-Ravanal, R., Lobos-González, L., Huilcamán, R., González, M. F., Díaz, J., Verschae, A. C., Acevedo, J. P., Carrasco, M., Sepúlveda, F., Jeldes, E., Varas-Godoy, M., Leyton, L., & Quest, A. F. (2023). Caveolin-1-dependent tenascin c inclusion in extracellular vesicles is required to promote breast cancer cell malignancy. Nanomedicine, 18(23), 1651-1668. https://doi.org/10.2217/nnm-2023-0143
Subjects
breast cancer
;
cav1
;
exosomes
;
metastasis
;
tnc
;
animals
;
breast neoplasms
;
caveolin 1
;
cell line, tumor
;
disease progression
;
extracellular vesicles
;
humans
;
mice
;
mice, scid
;
tenascin
;
caveolin 1
;
tenascin
;
cav1 protein, human
;
caveolin 1
;
tenascin
;
tnc protein, human
;
animal experiment
;
animal model
;
animal tissue
;
article
;
bagg albino mouse
;
cancer cell
;
cancer growth
;
cell invasion
;
cell migration
;
cell therapy
;
colony formation
;
controlled study
;
exosome
;
flow cytometry
;
human
;
human cell
;
in vitro study
;
in vivo study
;
mda-mb-231 cell line
;
metastatic breast cancer
;
nod scid mouse
;
nonhuman
;
protein expression
;
tumor xenograft
;
western blotting
;
animal
;
breast tumor
;
disease exacerbation
;
metabolism
;
mouse
;
pathology
;
scid mouse
;
tumor cell line