Annona cherimola Seed Extracts Trigger an Early Apoptosis Response and Selective Anticlonogenic Activity against the Human Gastric Carcinoma Cell Line SNU-1
Journal
Molecules
ISSN
1420-3049
Date Issued
2023
Author(s)
Johan Macuer-Guzmán
Claudia Giovagnoli-Vicuña
Giuliano Bernal
Lorena Lobos-González
Erwin de la Fuente-Ortega
Michael Araya-Castillo
Cristian Ibáñez
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:p>The aim of this study was to evaluate, for the first time, the antiproliferative, apoptotic and diminishing effects of the anchored growth-independent capacity of an ethanol macerate extract from the Annona cherimola seed (EMCHS) in the human gastric cancer cell line SNU-1. The cells treated with EMCHS (20 μg/mL) significantly reduced the capacity to form clones of the tumor cell. Moreover, 50 μg/mL of EMCHS extract induced apoptosis, as was shown by the Annexin-V assay. UHPLC-MS/MS analysis detected two acetogenins (Annonacinone and Annonacin) in the EMCHS, which could be largely responsible for its selective antiproliferative effect. The identification of fatty acids by GC-FID showed the presence of eight fatty acids, among which was, oleic acid, which has recognized activity as an adjuvant in antitumor treatments. Taken together, our results indicate that the EMCHS seems promising for use as a natural therapy against gastric cancer disease.</jats:p>
Cite this document
Macuer-Guzmán, J., Giovagnoli-Vicuña, C., Bernal, G., Lobos-González, L., De La Fuente-Ortega, E., Araya-Castillo, M., & Ibáñez, C. (2023). Annona cherimola seed extracts trigger an early apoptosis response and selective anticlonogenic activity against the human gastric carcinoma cell line snu-1. Molecules, 28(19), 6906. https://doi.org/10.3390/molecules28196906
Subjects
bioactivity
;
cancerous cells
;
ethanolic extract
;
fruit waste
;
acetogenins
;
annona
;
apoptosis
;
carcinoma
;
cell line
;
cell line, tumor
;
fatty acids
;
humans
;
plant extracts
;
seeds
;
stomach neoplasms
;
tandem mass spectrometry
;
acetogenin
;
fatty acid
;
plant extract
;
annona
;
apoptosis
;
carcinoma
;
cell line
;
human
;
plant seed
;
stomach tumor
;
tandem mass spectrometry
;
tumor cell line