SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level

Journal
Scientific Reports
ISSN
2045-2322
Date Issued
2021
Author(s)
Gina Pennacchiotti
Fabio Valdés-Gutiérrez
Wilfredo Alejandro González-Arriagada
Héctor Federico Montes
Judith Maria Roxana Parra
Valeria Andrea Guida
Silvina Esther Gómez
Martin Eduardo Guerrero-Gimenez
Juan Manuel Fernandez-Muñoz
Felipe Carlos Martin Zoppino
Rubén Walter Carón
Ricardo Fernández-Ramires
Flavia Alejandra Bruna
Type
Resource Types::text::journal::journal article
Scopus ID
2-s2.0-85103410096
WoS ID
WOS:000635702100022
DOI
URL
URL Institutional Repository
Abstract
<jats:title>Abstract</jats:title><jats:p>The oral squamous cell carcinoma (OSCC), which has a high morbidity rate, affects patients worldwide. Changes in SPINK7 in precancerous lesions could promote oncogenesis. Our aim was to evaluate SPINK7 as a potential molecular biomarker which predicts OSCC stages, compared to: HER2, TP53, RB1, NFKB and CYP4B1. This study used oral biopsies from three patient groups: dysplasia (n = 33), less invasive (n = 28) and highly invasive OSCC (n = 18). The control group consisted of clinically suspicious cases later to be confirmed as normal mucosa (n = 20). Gene levels of <jats:italic>SPINK7, P53, RB, NFKB</jats:italic> and <jats:italic>CYP4B1</jats:italic> were quantified by qPCR. <jats:italic>SPINK7</jats:italic> levels were correlated with a cohort of 330 patients from the TCGA. Also, SPINK7, HER2, TP53, and RB1, were evaluated by immunohistofluorescence. One-way Kruskal–Wallis test and Dunn's <jats:italic>post-hoc</jats:italic> with a <jats:italic>p</jats:italic> < 0.05 significance was used to analyze data. In OSCC, the <jats:italic>SPINK7</jats:italic> expression had down regulated while <jats:italic>P53, RB, NFKB</jats:italic> and <jats:italic>CYP4B1</jats:italic> had up regulated (<jats:italic>p</jats:italic> < 0.001). <jats:italic>SPINK7</jats:italic> had also diminished in TCGA patients (<jats:italic>p</jats:italic> = 2.10e-6). In less invasive OSCC, SPINK7 and HER2 proteins had decreased while TP53 and RB1 had increased with respect to the other groups (<jats:italic>p</jats:italic> < 0.05). The changes of SPINK7 accompanied by HER2, P53 and RB1 can be used to classify the molecular stage of OSCC lesions allowing a diagnosis at molecular and histopathological levels.
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Cite this document
Pennacchiotti, G., Valdés-Gutiérrez, F., González-Arriagada, W. A., Montes, H. F., Parra, J. M. R., Guida, V. A., Gómez, S. E., Guerrero-Gimenez, M. E., Fernandez-Muñoz, J. M., Zoppino, F. C. M., Carón, R. W., Ezquer, M. E., Fernández-Ramires, R., & Bruna, F. A. (2021). SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level. Scientific Reports, 11(1), 6939. https://doi.org/10.1038/s41598-021-86208-z
Subjects
adult

; 

biomarkers, tumor

; 

carcinoma, squamous cell

; 

case-control studies

; 

female

; 

humans

; 

male

; 

middle aged

; 

mouth neoplasms

; 

receptor, erbb-2

; 

retinoblastoma binding proteins

; 

serine peptidase inhibitors, kazal type

; 

tumor suppressor protein p53

; 

ubiquitin-protein ligases

; 

epidermal growth factor receptor 2

; 

erbb2 protein, human

; 

protein p53

; 

rb1 protein, human

; 

retinoblastoma binding protein

; 

serine peptidase inhibitor kazal type

; 

spink7 protein, human

; 

tumor marker

; 

ubiquitin protein ligase

; 

adult

; 

case control study

; 

female

; 

genetics

; 

human

; 

male

; 

metabolism

; 

middle aged

; 

mouth tumor

; 

squamous cell carcinoma