Mucosal Vaccination with Lactococcus lactis-Secreting Surface Immunological Protein Induces Humoral and Cellular Immune Protection against Group B Streptococcus in a Murine Model
Journal
Vaccines
ISSN
2076-393X
Date Issued
2020
Author(s)
Diego A. Diaz-Dinamarca
Carlos Hernandez
Daniel F. Escobar
Daniel A. Soto
Guillermo A. Muñoz
Jesús F. Badilla
Ricardo A. Manzo
Alexis M. Kalergis
Abel E. Vasquez
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:p>Group B Streptococcus (GBS) is the primary etiological agent of sepsis and meningitis in newborns and is associated with premature birth and stillbirth. The development of a licensed vaccine is one of the pending challenges for the World Health Organization. Previously, we showed that oral immunization with surface immune protein (SIP) decreases vaginal colonization of GBS and generates functional opsonizing antibodies, which was determined by opsonophagocytic assays (OPA) in vitro. We also showed that the protein has an adjuvant vaccine profile. Therefore, an oral vaccine based on SIP may be an attractive alternative to employ in the development of new vaccines against GBS. Lactococcus lactis is a highlighted oral vaccine probiotic inducer of the mucosal immune response. This bacterium could serve as an antigen-delivering vehicle for the development of an edible vaccine and has been used in clinical trials. In this study, we showed that an oral vaccine with a recombinant L. lactis strain secreting SIP from GBS (rL. lactis-SIP) can induce protective humoral and cellular immunity in an experimental model of GBS vaginal colonization in C57BL/6 mice. Mice immunized with rL. lactis-SIP were protected against clinical symptoms and bacterial colonization after GBS vaginal colonization. Our rL. lactis-SIP vaccine also induces an increase of immunoglobulin G (IgG) and immunoglobulin A (IgA) specifically against SIP. The adoptive transfer of serum from vaccinated mice to naïve mice generated protection against GBS vaginal colonization. Moreover, the rL. lactis-SIP strain induces the activation of SIP-specific T cells, which could decrease GBS vaginal colonization and generate protective antibodies when transferred to other mice. Our experimental observations strongly support the notion that rL. lactis-SIP induces protective humoral and cellular immunity and could be considered as a novel alternative in the development of vaccines for GBS.</jats:p>
Cite this document
Diaz-Dinamarca, D. A., Hernandez, C., Escobar, D. F., Soto, D. A., Muñoz, G. A., Badilla, J. F., Manzo, R. A., Carrión, F., Kalergis, A. M., & Vasquez, A. E. (2020). Mucosal vaccination with lactococcus lactis-secreting surface immunological protein induces humoral and cellular immune protection against group b streptococcus in a murine model. Vaccines, 8(2), 146. https://doi.org/10.3390/vaccines8020146
Subjects
cellular immune response
;
group b streptococcus
;
humoral immune response
;
mucosal vaccine
;
surface immunogenic protein
;
bacterial vaccine
;
immunoglobulin
;
immunoglobulin a
;
immunoglobulin g
;
adoptive transfer
;
animal experiment
;
animal model
;
article
;
bacterial colonization
;
bacterium transformation
;
bioassay
;
cell differentiation
;
cell stimulation
;
cellular immunity
;
colony forming unit
;
controlled study
;
enzyme linked immunosorbent assay
;
escherichia coli
;
female
;
flow cytometry
;
gene expression
;
human
;
human cell
;
humoral immunity
;
immune response
;
immunization
;
immunogenicity
;
lactococcus lactis
;
mouse
;
nonhuman
;
nucleotide sequence
;
opsonophagocytic assay
;
protein expression
;
protein secretion
;
real time polymerase chain reaction
;
regulatory t lymphocyte
;
spleen cell
;
streptococcus agalactiae
;
t lymphocyte activation
;
vaccination
;
western blotting