Peptide Targeted Gold Nanoplatform Carrying miR-145 Induces Antitumoral Effects in Ovarian Cancer Cells
Journal
Pharmaceutics
ISSN
1999-4923
Date Issued
2022
Author(s)
Edison Salas-Huenuleo
Andrea Hernández
Lorena Lobos-González
Iva Polakovičová
Francisco Morales-Zavala
Eyleen Araya
Freddy Celis
Carmen Romero
Marcelo J. Kogan
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:p>One of the recent attractive therapeutic approaches for cancer treatment is restoring downregulated microRNAs. They play an essential muti-regulatory role in cellular processes such as proliferation, differentiation, survival, apoptosis, cell cycle, angiogenesis, and metastasis, among others. In this study, a gold nanoplatform (GNPF) carrying miR-145, a downregulated microRNA in many cancer types, including epithelial ovarian cancer, was designed and synthesized. For targeting purposes, the GNPF was functionalized with the FSH33 peptide, which provided selectivity for ovarian cancer, and loaded with the miR-145 to obtain the nanosystem GNPF-miR-145. The GNPF-mir-145 was selectively incorporated in A2780 and SKOV3 cells and significantly inhibited cell viability and migration and exhibited proliferative and anchor-independent growth capacities. Moreover, it diminished VEGF release and reduced the spheroid size of ovarian cancer through the damage of cell membranes, thus decreasing cell viability and possibly activating apoptosis. These results provide important advances in developing miR-based therapies using nanoparticles as selective vectors and provide approaches for in vivo evaluation.</jats:p>
Cite this document
Salas-Huenuleo, E., Hernández, A., Lobos-González, L., Polakovičová, I., Morales-Zavala, F., Araya, E., Celis, F., Romero, C., & Kogan, M. J. (2022). Peptide targeted gold nanoplatform carrying mir-145 induces antitumoral effects in ovarian cancer cells. Pharmaceutics, 14(5), 958. https://doi.org/10.3390/pharmaceutics14050958
Subjects
nanoparticles
;
degradation
;
apoptosis
;
growth
;
proliferation
;
metastasis
;
scattering
;
micrornas
;
delivery
;
invasion
;
carboplatin
;
gold nanoparticle
;
lactate dehydrogenase
;
lipocortin 5
;
microrna 145
;
paclitaxel
;
vasculotropin
;
absorption spectrophotometry
;
angiogenesis
;
antineoplastic activity
;
antiproliferative activity
;
apoptosis
;
apoptosis assay
;
article
;
atomic emission spectrometry
;
cancer therapy
;
cell cycle
;
cell differentiation
;
cell migration
;
cell proliferation
;
cell proliferation assay
;
cell survival
;
cell viability
;
chemotherapy
;
clonogenic assay
;
colorimetry
;
controlled study
;
down regulation
;
electrophoretic mobility
;
human
;
human cell
;
metastasis
;
mts assay
;
ovary cancer
;
ovary carcinoma
;
photon correlation spectroscopy
;
physical chemistry
;
propidium iodide assay
;
raman spectrometry
;
real time polymerase chain reaction
;
scanning transmission electron microscopy
;
wound healing assay
;
zeta potential