Renal peritumoral adipose tissue undergoes a browning process and stimulates the expression of epithelial-mesenchymal transition markers in human renal cells

Journal
Scientific Reports
ISSN
2045-2322
Date Issued
2022
Author(s)
Matías Ferrando
Flavia Alejandra Bruna
Leonardo Rafael Romeo
Daiana Lorena Moya-Morales
Flavia Santiano
Leila Zyla
Silvina Gomez
Constanza Matilde Lopez-Fontana
Juan Carlos Calvo
Rubén Walter Carón
Judith Toneatto
Virginia Pistone-Creydt
Type
Resource Types::text::journal::journal article
Scopus ID
2-s2.0-85130499925
WoS ID
WOS:000799388600005
DOI
URL
URL Institutional Repository
Abstract
<jats:title>Abstract</jats:title><jats:p>Tumor cells can interact with neighboring adipose cells and adipocyte dedifferentiation appears to be an important aspect of tumorigenesis. We evaluated the size of adipocytes in human adipose explants from normal (hRAN) and kidney cancer (hRAT); changes in the expression of WAT and BAT/beige markers in hRAN and hRAT; the expression of epithelial-mesenchymal transition (EMT) cell markers in human kidney tumor (786-O, ACHN and Caki-1); and non-tumor (HK-2) epithelial cell lines incubated with the conditioned media (CMs) of hRAN and hRAT. We observed that hRAT adipocytes showed a significantly minor size compared to hRAN adipocytes. Also, we observed that both Prdm16 and Tbx1 mRNA and the expression of UCP1, TBX1, PPARγ, PCG1α, c/EBPα LAP and c/EBPα LIP was significantly higher in hRAT than hRAN. Finally, we found an increase in vimentin and N-cadherin expression in HK-2 cells incubated for 24 h with hRAT-CMs compared to hRAN- and control-CMs. Furthermore, desmin and N-cadherin expression also increased significantly in 786-O when these cells were incubated with hRAT-CMs compared to the value observed with hRAN- and control-CMs. We observed a significant decrease in E-cadherin expression in the ACHN cell line incubated with hRAT-CMs versus hRAN- and control-CMs. However, we did not observe changes in E-cadherin expression in HK-2, 786-O or Caki-1. The results obtained, together with the results previously published by our group, allow us to conclude that perirenal white adipose tissue browning contributes to tumor development in kidney cancer. In addition, hRAT-CMs increases the expression of mesenchymal markers in renal epithelial cells, which could indicate a regulation of EMT due to this adipose tissue.
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Cite this document
Ferrando, M., Bruna, F. A., Romeo, L. R., Contador, D., Moya-Morales, D. L., Santiano, F., Zyla, L., Gomez, S., Lopez-Fontana, C. M., Calvo, J. C., Carón, R. W., Toneatto, J., & Pistone-Creydt, V. (2022). Renal peritumoral adipose tissue undergoes a browning process and stimulates the expression of epithelial-mesenchymal transition markers in human renal cells. Scientific Reports, 12(1), 8687. https://doi.org/10.1038/s41598-022-12746-9
Subjects
nuclear receptors

; 

adipocytes

; 

adipose tissue

; 

adipose tissue, brown

; 

adipose tissue, white

; 

cadherins

; 

epithelial-mesenchymal transition

; 

humans

; 

kidney neoplasms

; 

cadherin

; 

adipose tissue

; 

brown adipose tissue

; 

epithelial mesenchymal transition

; 

genetics

; 

human

; 

kidney tumor

; 

metabolism

; 

white adipose tissue