A Tetrameric Peptide Derived from Bovine Lactoferricin Exhibits Specific Cytotoxic Effects against Oral Squamous-Cell Carcinoma Cell Lines
Journal
BioMed Research International
ISSN
2314-6133
2314-6141
Date Issued
2015
Author(s)
Víctor A. Solarte
Jaiver E. Rosas
Zuly J. Rivera
Martha L. Arango-Rodríguez
Javier E. García
Jean-Paul Vernot
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:p>Several short linear peptides derived from cyclic bovine lactoferricin were synthesized and tested for their cytotoxic effect against the oral cavity squamous-cell carcinoma (OSCC) cell lines CAL27 and SCC15. As a control, an immortalized and nontumorigenic cell line, Het-1A, was used. Linear peptides based on the RRWQWR core sequence showed a moderate cytotoxic effect and specificity towards tumorigenic cells. A tetrameric peptide, LfcinB(20–25)<jats:sub>4</jats:sub>, containing the RRWQWR motif, exhibited greater cytotoxic activity (>90%) in both OSCC cell lines compared to the linear lactoferricin peptide or the lactoferrin protein. Additionally, this tetrameric peptide showed the highest specificity towards tumorigenic cells among the tested peptides. Interestingly, this effect was very fast, with cell shrinkage, severe damage to cell membrane permeability, and lysis within one hour of treatment. Our results are consistent with a necrotic effect rather than an apoptotic one and suggest that this tetrameric peptide could be considered as a new candidate for the therapeutic treatment of OSCC.</jats:p>
Subjects
inhibit tumor-metastasis
;
antitumor-activity
;
cancer-treatment
;
in-vitro
;
apoptosis
;
carcinogenesis
;
mice
;
management
;
neck
;
etiopathogenesis