Genomic Epidemiology of Vancomycin-Resistant Enterococcus faecium (VREfm) in Latin America: Revisiting The Global VRE Population Structure
Journal
Scientific Reports
ISSN
2045-2322
Date Issued
2020
Author(s)
Rafael Rios
Jinnethe Reyes
Lina P. Carvajal
Sandra Rincon
Diana Panesso
Aura M. Echeverri
An Dinh
Sergios-Orestis Kolokotronis
Apurva Narechania
Truc T. Tran
Barbara E. Murray
Paul J. Planet
Cesar A. Arias
Lorena Diaz
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:title>Abstract</jats:title><jats:p>Little is known about the population structure of vancomycin-resistant <jats:italic>Enterococcus faecium</jats:italic> (VR<jats:italic>Efm</jats:italic>) in Latin America (LATAM). Here, we provide a complete genomic characterization of 55 representative Latin American VR<jats:italic>Efm</jats:italic> recovered from 1998–2015 in 5 countries. The LATAM VR<jats:italic>Efm</jats:italic> population is structured into two main clinical clades without geographical clustering. Using the LATAM genomes, we reconstructed the global population of VR<jats:italic>Efm</jats:italic> by including 285 genomes from 36 countries spanning from 1946 to 2017. In contrast to previous studies, our results show an early branching of animal related isolates and a further split of clinical isolates into two sub-clades within clade A. The overall phylogenomic structure of clade A was highly dependent on recombination (54% of the genome) and the split between clades A and B was estimated to have occurred more than 2,765 years ago. Furthermore, our molecular clock calculations suggest the branching of animal isolates and clinical clades occurred ~502 years ago whereas the split within the clinical clade occurred ~302 years ago (previous studies showed a more recent split between clinical an animal branches around ~74 years ago). By including isolates from Latin America, we present novel insights into the population structure of VR<jats:italic>Efm</jats:italic> and revisit the evolution of these pathogens.</jats:p>
Cite this document
Rios, R., Reyes, J., Carvajal, L. P., Rincon, S., Panesso, D., Echeverri, A. M., Dinh, A., Kolokotronis, S.-O., Narechania, A., Tran, T. T., Munita, J. M., Murray, B. E., Planet, P. J., Arias, C. A., & Diaz, L. (2020). Genomic epidemiology of vancomycin-resistant enterococcus faecium (Vrefm) in latin america: Revisiting the global vre population structure. Scientific Reports, 10(1), 5636. https://doi.org/10.1038/s41598-020-62371-7
Subjects
anti-bacterial agents
;
cross infection
;
disease outbreaks
;
enterococcus faecium
;
genomics
;
genotype
;
gram-positive bacterial infections
;
humans
;
microbial sensitivity tests
;
molecular epidemiology
;
phylogeny
;
vancomycin
;
vancomycin-resistant enterococci
;
antiinfective agent
;
vancomycin
;
article
;
calculation
;
cladistics
;
infectious agent
;
molecular clock
;
nonhuman
;
population structure
;
south and central america
;
vancomycin resistant enterococcus
;
cross infection
;
drug effect
;
enterococcus faecium
;
epidemic
;
genetics
;
genomics
;
genotype
;
gram positive infection
;
human
;
microbial sensitivity test
;
microbiology
;
molecular epidemiology
;
phylogeny
;
procedures
;
vancomycin resistant enterococcus