PORTE TORRE, LORENA ISABEL
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PORTE TORRE, LORENA ISABEL
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lporte@udd.cl
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Head-to-head comparison of CAMPYAIR aerobic culture medium versus standard microaerophilic culture for Campylobacter isolation from clinical samples

2023 , Arturo Levican , Carmen Varela , Lorena Porte , WEITZEL, THOMAS , Isabel Briceño , Francisco Guerra , Benjamín Mena , Arthur Hinton

Campylobacter spp. are considered the most frequent cause of acute gastroenteritis worldwide. However, outside high-income countries, its burden is poorly understood. Limited published data suggest that Campylobacter prevalence in low- and middle-income countries is high, but their reservoirs and age distribution are different. Culturing Campylobacter is expensive due to laboratory equipment and supplies needed to grow the bacterium (e.g., selective culture media, microaerophilic atmosphere, and a 42°C incubator). These requirements limit the diagnostic capacity of clinical laboratories in many resource-poor regions, leading to significant underdiagnosis and underreporting of isolation of the pathogen. CAMPYAIR, a newly developed selective differential medium, permits Campylobacter isolation without the need for microaerophilic incubation. The medium is supplemented with antibiotics to allow Campylobacter isolation in complex matrices such as human feces. The present study aims to evaluate the ability of the medium to recover Campylobacter from routine clinical samples. A total of 191 human stool samples were used to compare the ability of CAMPYAIR (aerobic incubation) and a commercial Campylobacter medium (CASA, microaerophilic incubation) to recover Campylobacter. All Campylobacter isolates were then identified by MALDI-TOF MS. CAMPYAIR showed sensitivity and specificity values of 87.5% (95% CI 47.4%–99.7%) and 100% (95% CI 98%–100%), respectively. The positive predictive value of CAMPYAIR was 100% and its negative predictive value was 99.5% (95% CI 96.7%–99.9%); Kappa Cohen coefficient was 0.93 (95% CI 0.79–1.0). The high diagnostic performance and low technical requirements of the CAMPYAIR medium could permit Campylobacter culture in countries with limited resources.

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Creación del primer biorrepositorio nacional de bacterias multirresistentes disponible para el estudio de la resistencia bacteriana en Chile

2022 , Patricia García , RIVAS JIMENEZ, LINA MARIA , Anne Peters , Paola Henríquez , Loriana Castillo , Vijna Illesca , Andrea Maripani , Juan Moreno , Margareta Mühlhauser , PORTE TORRE, LORENA ISABEL , María Luisa Rioseco , Pamela Rojas , Francisco Silva , Patricio Suazo , MUNITA SEPULVEDA, JOSE MANUEL

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Genetic diversity and clonal characteristics of ciprofloxacin-resistant Campylobacter jejuni isolated from Chilean patients with gastroenteritis

2018 , Luis Collado , Nataly Muñoz , Lorena Porte , Sofía Ochoa , Carmen Varela , Ivo Muñoz

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Bacteriemia por Vibrio cholerae no-O1/no-O139 que porta una región homóloga a la isla de patogenicidad VpaI-7

2019 , Felipe Olivares , Ignacio Domínguez , Jeannette Dabanch , PORTE TORRE, LORENA ISABEL , M. Teresa Ulloa , Gonzalo Osorio

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Longitudinal study of wound healing status and bacterial colonisation of Staphylococcus aureus and Corynebacterium diphtheriae in epidermolysis bullosa patients

2022 , FUENTES BUSTOS, MARIA IGNACIA , YUBERO GONCALVEZ, MARIA JOAO , Pilar Morandé , Carmen Varela , Karen Oróstica , Francisco Acevedo , REBOLLEDO JARAMILLO, BORIS EDUARDO , Esteban Arancibia , PORTE TORRE, LORENA ISABEL , PALISSON ETCHARREN, FRANCIS

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Corynebacterium kroppenstedtii

2012 , PORTE TORRE, LORENA ISABEL , WEITZEL, THOMAS

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Genomic Analysis of Chilean Strains of Campylobacter jejuni from Human Faeces

2019 , Arturo Levican , Ignacio Ramos-Tapia , Isabel Briceño , Francisco Guerra , Benjamin Mena , Carmen Varela , PORTE TORRE, LORENA ISABEL

Campylobacterspp., especiallyC. jejuni, are recognized worldwide as the bacterial species that most commonly cause food-related diarrhea.C. jejunipossesses many different virulence factors, has the ability to survive in different reservoirs, and has shown among isolates the emergence of Antimicrobial Resistance (AMR). Genome association analyses of this bacterial pathogen have contributed to a better understanding of its pathogenic and AMR associated determinants. However, the epidemiological information of these bacteria in Latin American countries is scarce and no genomic information is available in public databases from isolates in these countries. Considering this, the present study is aimed to describe the genomic traits from representativeCampylobacterspp. strains recovered from faecal samples of patients with acute diarrhoea from Valparaíso, Chile.Campylobacterspp. was detected from the faeces of 28 (8%) out of 350 patients with acute diarrhoea, mainly from young adults and children, and 26 (93%) of the isolates corresponded toC. jejuni. 63% of the isolates were resistant to ciprofloxacin, 25.9% to tetracycline, and 3.5% to erythromycin. Three isolates were selected for WGS on the basis of theirflaA-RFLP genotype. They belonged to the multilocus sequence typing (MLST) clonal clomplex (CC) 21(PUCV-1), CC-48 (PUCV-3), and CC-353 (PUCV-2) and presented several putative virulence genes, including the Type IV and Type VI Secretion Systems, as well as AMR-associated genes in agreement with their susceptibility pattern. On the basis of the wgMLST, they were linked to strains from poultry and ruminants. These are the first genomes of ChileanC. jejuniisolates available in public databases and they provide relevant information about theC. jejuniisolates associated with human infection in this country.

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Draft Whole-Genome Sequences of 51 Campylobacter jejuni and 12 Campylobacter coli Clinical Isolates from Chile

2020 , Verónica Bravo , Carmen Varela , PORTE TORRE, LORENA ISABEL , WEITZEL, THOMAS , George J. Kastanis , Maria Balkey , Carlos J. Blondel , Narjol Gonzalez-Escalona , David Rasko

Campylobacter species are the leading cause of gastroenteritis worldwide and an emerging threat in developing countries. Here, we report the draft whole-genome sequences of 51 Campylobacter jejuni and 12 Campylobacter coli strains isolated from patients with gastroenteritis in Santiago, Chile.

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Chilean strains of clinical origin of non-O1, non-O139 Vibrio cholerae carry the genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF from secretion system T3SS2 present in an island of pathogenicity

2019 , María Teresa Ulloa , Camila Sanhueza , Tania Henríquez , Benjamín Aguayo , Germán Hermosilla , PORTE TORRE, LORENA ISABEL , Jeannette Dabanch , Stephanie Braun , Alberto Fica , Isabel Briceño , Carlos Gonzalo Osorio

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Atypical mycobacterial infections in five adult patients without evidence of immunosuppression. Making an experience

2015 , Alberto Fica , Andrés Soto , Jeannette Dabanch , PORTE TORRE, LORENA ISABEL , CASTRO DOMINGUEZ, MARIO MARCELO , M. Elvira Balcells , THOMPSON MOYA, LUIS ADALBERTO

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