NUÑEZ VASQUEZ, GONZALO ESTEBAN
Preferred name
NUÑEZ VASQUEZ, GONZALO ESTEBAN
Main Affiliation
Email
nunezgonzalo89@gmail.com
ORCID
0000-0002-6698-1732
Scopus Author ID
60083737100, 59715362600
3 results
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Item type:Publication, Connexin46 in the nucleus of cancer cells: a possible role as transcription modulator(Springer Science and Business Media LLC, 2025-03-27); Pablo Pérez-MorenoScopus© Citations 1 10 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, KFERQ-selective protein autophagy in Caenorhabditis elegans depends on LMP-1(Public Library of Science (PLoS), 2025-09-02); ; Carlos F. LagosMammalian cells exhibit three autophagy mechanisms: macroautophagy, microautophagy (MIA), and chaperone-mediated autophagy (CMA), each employing unique mechanisms for transporting cellular material to the lysosome for degradation. MIA involves the engulfment of proteins via lysosomes/late endosomes through membrane invagination, while CMA directly imports cytosolic proteins into lysosomes, selectively targeting those harboring the KFERQ pentapeptide motif, helped by the chaperone HSC70. Despite the identification of several genetic markers of these pathways, our understanding of the underlying mechanisms, particularly in MIA and CMA, remains limited. To study CMA in vivo we designed a photoactivatable CMA reporter consisting of a plasmid encoding the KFERQ consensus signal for CMA targeting. We generated transgenic <jats:italic><jats:italic>C. elegans</jats:italic></jats:italic> strains with diverse genetic backgrounds to analyze the role of known molecular components of CMA in mammals. Additionally, we conducted an in-silico analysis of the structural interaction between <jats:italic><jats:italic>C. elegans</jats:italic></jats:italic> LMP-1 or LMP-2 proteins with the HSP-1 chaperone. Results: Our study shows a significant alteration in the distribution pattern of the KFERQ reporter in muscle cells upon induction of selective autophagy (CMA or MIA). We found that the reporter localized into lysosomes only during starvation, which abrogated in the absence of LMP-1. This study validates CMA in <jats:italic><jats:italic>C. elegans</jats:italic></jats:italic> and provides the development of a new tool for understanding selective autophagy mechanisms and their potential implications in various organisms.1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Pannexin-1 expression in tumor cells correlates with colon cancer progression and survival(2024)2Scopus© Citations 9