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    Concordance of Helicobacter pylori Detection Methods in Symptomatic Children and Adolescents
    (MDPI AG, 2025-03-04)
    Camila Cabrera
    ;
    Yanira Campusano
    ;
    Joaquín Torres
    ;
    Dinka Ivulic
    ;
    Valeria Galvez
    Background: Helicobacter pylori is the most prevalent chronic bacterial infection globally, acquired mostly during childhood. It is associated with chronic gastritis, peptic ulcer disease, and gastric cancer. Due to challenges in culturing H. pylori, diagnostic reference standards often rely on combining ≥2 non-culture, biopsy-based methods. Histology with Giemsa staining is widely used in clinical settings due to its low cost and reliable performance. Methods: This study evaluated the concordance between histology with Giemsa staining as the reference standard and other diagnostic methods, including the rapid urease test (RUT), ureA RT-PCR, 16S sequencing, and anti-H. pylori serum IgG. Positive percent of agreement (PPA), negative percent of agreement (NPA) and concordance kappa index were calculated. Results: A total of 120 patients (41 positive and 79 negative by Giemsa staining) were analyzed. Among the methods tested, RT-PCR for ureA showed the best performance (PPA = 94.7%, NPA = 98.6%, kappa = 0.939), while RUT underperformed compared with expectations (PPA = 65.9%, NPA = 97.5%, kappa = 0.681). Serology had the lowest performance (PPA = 53.7%, NPA = 96.1%, kappa = 0.548). Conclusions: The combination of histology with Giemsa staining and ureA RT-PCR achieved the highest detection rate and strongest agreement.</jats:p>
      1
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    Paw Skin as a Translational Model for Investigating Fibrotic and Inflammatory Wound Healing Defects in Recessive Dystrophic Epidermolysis Bullosa
    (MDPI AG, 2025-04-30)
    DE GREGORIO CONCHA, CRISTIAN ALEJANDRO  
    ;
    Giselle Ramos-Gonzalez
    ;
    Bernardo Morales-Catalán
    ;
    EZQUER, EDUARDO FERNANDO  
    ;
    EZQUER, EDUARDO MARCELO  
    Recessive dystrophic epidermolysis bullosa (RDEB) is a severe genetic disease caused by COL7A1 mutations. It leads to skin fragility, chronic inflammation, and impaired wound healing. The condition often results in fibrotic scarring, pseudosyndactyly, and cutaneous squamous cell carcinoma (SCC). However, current animal models fail to fully replicate chronic RDEB wounds. In this study, we used Collagen VII-hypomorphic mice (Col7a1flNeo/flNeo) and created full-thickness wounds on their paw skin, an area prone to fibrosis due to mechanical stress. We analyzed the healing process using histology, immunofluorescence, and electron microscopy. The RDEB mice showed delayed wound closure, increased inflammation, and poor granulation tissue formation. At 30 days post-injury, we observed persistent fibrosis, with elevated levels of Collagen I, α-SMA+ myofibroblasts, and tenascin-C. These mice also had fewer intraepidermal nerve fibers, which may help explain the neuropathic pain associated with RDEB. Our model reproduces the main features of chronic RDEB wounds. It offers a useful tool for evaluating therapies aimed at reducing inflammation, fibrosis, and tumor risk in these patients.
    Scopus© Citations 3  4
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    Prenatal presentation of pleuropulmonary blastoma associated to DICER1 syndrome: differential diagnosis of congenital pulmonary malformation
    (2023)
    Valenzuela, Catalina Catán
    ;
    Paula Vargas Innocenti
    ;
    Gutierrez, Aquiles Hachim
    ;
    Pinto, Pablo Jorquera
    ;
    Ramos, Ximena Claverie
      3Scopus© Citations 2
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    Polycaprolactone-based scaffolds for guided tissue regeneration in periodontal therapy: A systematic review
    (2023)
    Florencia Antunovic
    ;
    Felipe Tolosa
    ;
    Catherine Klein
    ;
    René Ocaranza
    <jats:sec><jats:title>Background:</jats:title><jats:p> Polycaprolactone (PCL) is a highly recognized synthetic polymer for its biocompatibility, ease of fabrication and mechanical strength in bone tissue engineering. Its applications have extended broadly, including regeneration of oral and maxillofacial lost tissues. Its usefulness has brought attention of researchers to regenerate periodontal lost tissues, including alveolar bone, periodontal ligament and cementum. The aim of this systematic review was to obtain an updated analysis of the contribution of PCL-based scaffolds in the alveolar bone regeneration process. </jats:p></jats:sec><jats:sec><jats:title>Methods:</jats:title><jats:p> This review adheres to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines for systematic reviews. A computerized search of the PubMed, EBSCO, Scielo and Web of Science databases was performed, restricting literature search to published studies in English or Spanish between January 2002 and March 2023. Database search returned 248 studies which were screened based on title, author names and publication dates. </jats:p></jats:sec><jats:sec><jats:title>Results:</jats:title><jats:p> Data from 17 studies were reviewed and tabulated. All studies combined PCL with other biomaterials (such as Alginate, hydroxyapatite, bioactive glass, poly (lactic-co-glycolic acid)), growth factors (BMP-2, rhCEMP1), and/or mesenchymal stromal cells (adipose-derived, bone marrow, periodontal ligament or gingiva mesenchymal stromal cells). PCL scaffolds showed higher cell viability and osteoinductive potential when combined with bioactive agents. Complementary, its degradation rates were affected by the addition or exposure to specific substances, such as: Dopamine, Cerium Oxide, PLGA and hydrogen peroxide. </jats:p></jats:sec><jats:sec><jats:title>Conclusions:</jats:title><jats:p> PCL is an effective biomaterial for alveolar bone regeneration in periodontally affected teeth. It could be part of a new generation of biomaterials with improved regenerative potential. </jats:p></jats:sec>
    Scopus© Citations 15
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      1Scopus© Citations 3  1
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    Incidence of occult uterine sarcoma and other unexpected pathologies in patients having surgery for presumed myomas: A retrospective observational study
    (2021)
    Mariane Von Mühlenbrock
    ;
    Paz Navarrete-Rey
    ;
    Elias Kovoor
    ;
    Rodrigo Guzman-Rojas
    ;
    Fernando Troncoso
      5Scopus© Citations 4
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    Lupus eritematoso sistémico buloso: Una manifestación infrecuente en población pediátrica
    (2021)
    María Trinidad Hasbún Z.
    ;
    ROLLAN MARZOLO, MARIA PAZ  
    ;
    Ximena Chaparro R.
    ;
    Cecilia Fischer S.
    ;
    Adriana Castrillón V.
    <jats:p>El lupus eritematoso sistémico buloso (LESB) es una enfermedad ampollar subepidérmica autoinmune secundaria a la presencia de autoanticuerpos contra el colágeno VII de la membrana basal. Es considerada una variante de lupus eritematoso sistémico (LES), siendo infrecuente en la población pediátrica.Objetivo: Describir caso de paciente pediátrica con erupción ampollar compatible con LESB.Caso Clínico: Paciente de sexo femenino de 16 años de ascendencia mapuche, con antecedentes de LES diagnosticado a los 10 años de edad, en tratamiento. Consultó por erupción vesiculobulosa generalizada de 6 semanas de evolución, sin sintomatología sistémica. Se realizó biopsia para estudio histológico e inmunofluorescencia directa (IFD), confirmándose el diagnóstico de LESB. La paciente respondió favorablemente al tratamiento con dapsona en dosis de 100 mg/día (asociado a su tratamiento de base), sin nuevas reactivaciones a 8 años de seguimiento.Conclusión: El LESB es una manifestación infrecuente de LES. Aunque la clínica es similar a la de otras dermatosis ampollares, la correlación entre la presencia de LES, los hallazgos histopatológicos y de IFD permiten confirmar el diagnóstico. Si bien se ha reportado mayor riesgo de LES en población de ascendencia indígena, faltan estudios respecto a la asociación de LESB en esta etnia.</jats:p>
    Scopus© Citations 2  1
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    Tumor-like presentation of herpetic cervicitis: A case report
    (2023)
    Pablo Boldrini L.
    ;
    Gabriel Vallejos P.
    ;
    Polentze Ballesteros P.
    ;
    Gonzalo Valenzuela L.
    ;
    Enrique Roncone D.
      2Scopus© Citations 1
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      1Scopus© Citations 3
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    DIVERTICULITIS APENDICULAR, REPORTE DE UN CASO
    (2022)
    José Miguel Campero Martínez
    ;
    Camila Cifuentes Jimenez
    ;
    Danieka Espinola Marin
    <jats:p>Objetivo: Presentar un caso de diverticulitis apendicular y compararlo con la literatura actual.Material y método: Registro clínico de un paciente que ingresa a urgencias del Hospital Padre Hurtado, incluyendo cuadro clínico, imagenología, manejo quirúrgico y anatomía patológica.Resultados: Paciente se presenta con cuadro de dolor abdominal atípico, con imagen sugerente de apendicitis diverticular. En pabellón se logra completar apendicectomía laparoscópica con buena evolución posterior. Al estudio patológico se confirman características histológicas de diverticulitis perforada apendicular.Discusión: Se presenta un cuadro clínico que se condice con lo descrito en la literatura actual, aportando imágenes características tanto de radiología como histopatología.Conclusión: Debido a su mayor riesgo de perforación y mortalidad, la diverticulitis apendicular es una patología que debe considerarse en los diagnósticos diferenciales de dolores abdominales atípicos, en hombres mayores de 30 años, especialmente con los hallazgos imagenológicos característicos.</jats:p>
      6