Universidad del Desarrollo

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Biofilm formation on collagen substrates modulates <i>Streptococcus mutans</i> bacterial extracellular nanovesicle production and cargo
(Royal Society of Chemistry (RSC), 2025)
Camila Leiva-Sabadini
;
Pablo Berríos
;
Paula Saavedra
;
Javiera Carrasco-Rojas
;
José Vicente González-Aramundiz
Streptococcus mutans is the major microbial etiological agent of dental caries and can adhere to surfaces such as type-I collagen, which is present in dentin and periodontal tissues. Recent studies have characterized planktonic S. mutans bacterial extracellular vesicles (bEVs) at the nanoscale range and demonstrated environmental-induced changes due to sugar presence or pH alterations. However, to date, no studies have explored whether surface-derived changes can modulate bEV production in the context of oral biofilm formation in the elderly. Therefore, this work aimed to determine the role of biofilm formation and collagen glycation on the nanoscale morphology and proteomic composition of S. mutans bEVs. For this, bEVs from S. mutans biofilms on native and glycated collagen surfaces were isolated, characterized, and compared to bEVs from planktonic cells. Nanoparticle tracking analysis (NTA), atomic force microscopy (AFM), and electron microscopy confirmed bEV production and showed that bEVs from biofilms are smaller in size and less abundant than those from planktonic cells. Furthermore, proteome analysis revealed that S. mutans biofilm formation on native and glycated collagen led to the enrichment of several key virulence proteins. Also, a shift towards proteins involved in metabolic processes was found in bEVs following biofilm formation on collagen surfaces, whereas glucan metabolism proteins were overexpressed in vesicles from the planktonic state. These results demonstrate that biofilm formation, as well as the glycation of collagen associated with aging and hyperglycaemia, can modulate bEV characteristics and cargo and could play a central role in S. mutans virulence and the development of diseases such as dental caries and periodontal disease.
2Scopus© Citations 4
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Royal jelly derived extracellular vesicles modulate microglial nanomechanics and inflammatory responses
(Royal Society of Chemistry (RSC), 2025)
Gabriela Zavala
;
Pablo Berríos
;
Felipe Sandoval
;
Graciela Bravo
;
Nelson P. Barrera
Royal jelly-derived extracellular vesicles counteract inflammation-induced mechanobiological alterations in microglia, restoring their mechanical properties and offering a novel interkingdom approach to modulate neuroinflammation.
Scopus© Citations 1 3
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Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging
(2024)
Simon Álvarez
;
Jose Morales
;
Paola Tiozzo-Lyon
;
Pablo Berrios
;
Valentina Barraza
<jats:p>Our study aimed to develop a novel <jats:italic>in vitro</jats:italic> microfabricated biomimetic dentin surface that simulates the complex surface microarchitecture of exposed dentin, as well as age-derived glycation of teeth, for the growth of polymicrobial oral biofilms.</jats:p>
7
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Aloe vera peel-derived nanovesicles display anti-inflammatory properties and prevent myofibroblast differentiation
(2024)
RAMIREZ PRADA, ORLANDO JOSUÉ
;
Florencia Pomareda
;
OLIVARES, MARIA BELEN
;
HUANG, YA LIN
;
Gabriela Zavala
18Scopus© Citations 59
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Modulation of the biophysical and biochemical properties of collagen by glycation for tissue engineering applications
(2023)
Mina Vaez
;
Meisam Asgari
;
Liisa Hirvonen
;
Gorkem Bakir
;
Emilie Khattignavong
5 1Scopus© Citations 21
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Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses
(2023)
Simón Álvarez
;
Pamina Contreras-Kallens
;
Sebastian Aguayo
;
Ramírez Prada, Orlando
;
Catalina Vallejos
1Scopus© Citations 17 4
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Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles
(2020)
Orlando J. Ramírez
;
Simón Alvarez
;
Pamina Contreras-Kallens
;
Nelson P. Barrera
;
Sebastian Aguayo
7Scopus© Citations 34
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Antibacterial Effect of Honey-Derived Exosomes Containing Antimicrobial Peptides Against Oral Streptococci
(2021)
Camila Leiva-Sabadini
;
Simon Alvarez
;
Nelson P Barrera
;
Christina MAP Schuh
;
Sebastian Aguayo
Scopus© Citations 39 3
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Ultrastructural characterisation of young and aged dental enamel by atomic force microscopy
(2022)
Camila Leiva‐Sabadini
;
Christina MAP Schuh
;
Nelson P. Barrera
;
Sebastian Aguayo
37Scopus© Citations 6
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Bacterial adhesion to collagens: implications for biofilm formation and disease progression in the oral cavity
(2021)
Simón Álvarez
;
Camila Leiva-Sabadini
;
Christina M. A. P. Schuh
;
Sebastian Aguayo
Scopus© Citations 26 4

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