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Access type: UnknownAuthor: search.filters.author.Apurva Narechania

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    Genomic Epidemiology of Vancomycin-Resistant Enterococcus faecium (VREfm) in Latin America: Revisiting The Global VRE Population Structure
    (2020)
    Rafael Rios
    ;
    Jinnethe Reyes
    ;
    Lina P. Carvajal
    ;
    Sandra Rincon
    ;
    Diana Panesso
    <jats:title>Abstract</jats:title><jats:p>Little is known about the population structure of vancomycin-resistant <jats:italic>Enterococcus faecium</jats:italic> (VR<jats:italic>Efm</jats:italic>) in Latin America (LATAM). Here, we provide a complete genomic characterization of 55 representative Latin American VR<jats:italic>Efm</jats:italic> recovered from 1998–2015 in 5 countries. The LATAM VR<jats:italic>Efm</jats:italic> population is structured into two main clinical clades without geographical clustering. Using the LATAM genomes, we reconstructed the global population of VR<jats:italic>Efm</jats:italic> by including 285 genomes from 36 countries spanning from 1946 to 2017. In contrast to previous studies, our results show an early branching of animal related isolates and a further split of clinical isolates into two sub-clades within clade A. The overall phylogenomic structure of clade A was highly dependent on recombination (54% of the genome) and the split between clades A and B was estimated to have occurred more than 2,765 years ago. Furthermore, our molecular clock calculations suggest the branching of animal isolates and clinical clades occurred ~502 years ago whereas the split within the clinical clade occurred ~302 years ago (previous studies showed a more recent split between clinical an animal branches around ~74 years ago). By including isolates from Latin America, we present novel insights into the population structure of VR<jats:italic>Efm</jats:italic> and revisit the evolution of these pathogens.</jats:p>
      1Scopus© Citations 47  1
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      7  1
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    Methicillin-Susceptible, Vancomycin-Resistant Staphylococcus aureus, Brazil
    (2015)
    Diana Panesso
    ;
    Paul J. Planet
    ;
    Lorena Diaz
    ;
    Jean-Emmanuel Hugonnet
    ;
    Truc T. Tran
      1Scopus© Citations 42  1
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    Transferable Vancomycin Resistance in a Community-Associated MRSA Lineage
    (2014)
    Flávia Rossi
    ;
    Lorena Diaz
    ;
    Aye Wollam
    ;
    Diana Panesso
    ;
    Yanjiao Zhou
      11  1Scopus© Citations 153
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    A Prospective Cohort Multicenter Study of Molecular Epidemiology and Phylogenonnics of Staphylococcus aureus Bacterennia in Nine Latin American Countries
    (2017)
    Cesar A. Arias
    ;
    Jinnethe Reyes
    ;
    Lina Paola Carvajal
    ;
    Sandra Rincon
    ;
    Lorena Diaz
    <jats:title>ABSTRACT</jats:title> <jats:p> <jats:named-content content-type="genus-species">Staphylococcus aureus</jats:named-content> is an important pathogen causing a spectrum of diseases ranging from mild skin and soft tissue infections to life-threatening conditions. Bloodstream infections are particularly important, and the treatment approach is complicated by the presence of methicillin-resistant <jats:named-content content-type="genus-species">S. aureus</jats:named-content> (MRSA) isolates. The emergence of new genetic lineages of MRSA has occurred in Latin America (LA) with the rise and dissemination of the community-associated USA300 Latin American variant (USA300-LV). Here, we prospectively characterized bloodstream MRSA recovered from selected hospitals in 9 Latin American countries. All isolates were typed by pulsed-field gel electrophoresis (PFGE) and subjected to antibiotic susceptibility testing. Whole-genome sequencing was performed on 96 MRSA representatives. MRSA represented 45% of all (1,185 <jats:named-content content-type="genus-species">S. aureus</jats:named-content> ) isolates. The majority of MRSA isolates belonged to clonal cluster (CC) 5. In Colombia and Ecuador, most isolates (≥72%) belonged to the USA300-LV lineage (CC8). Phylogenetic reconstructions indicated that MRSA isolates from participating hospitals belonged to three major clades. Clade A grouped isolates with sequence type 5 (ST5), ST105, and ST1011 (mostly staphylococcal chromosomal cassette <jats:italic>mec</jats:italic> [SCC <jats:italic>mec</jats:italic> ] I and II). Clade B included ST8, ST88, ST97, and ST72 strains (SCC <jats:italic>mec</jats:italic> IV, subtypes a, b, and c/E), and clade C grouped mostly Argentinian MRSA belonging to ST30. In summary, CC5 MRSA was prevalent in bloodstream infections in LA with the exception of Colombia and Ecuador, where USA300-LV is now the dominant lineage. Clonal replacement appears to be a common phenomenon, and continuous surveillance is crucial to identify changes in the molecular epidemiology of MRSA. </jats:p>
      7  1Scopus© Citations 101