Taro NagatomoJulio JiménezJute RichterSiegrid De BaereJeroen VanoirbeekGunnar NaulaersKarel AllegaertSiska CroubelsJan A. DeprestJaan Toelen2022-09-282022-09-282016http://hdl.handle.net/11447/1229https://investigadores.udd.cl/handle/123456789/321810.1159/0004429372-s2.0-84957899860WOS:000377632500006<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Caffeine is a commonly used drug for apnea of prematurity. It may, however, also have a beneficial effect on bronchopulmonary dysplasia (BPD), which is the most common complication of extreme preterm birth. &lt;b&gt;&lt;i&gt;Objectives:&lt;/i&gt;&lt;/b&gt; To study the inflammatory, structural and functional effects of caffeine in an animal model of BPD. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Preterm New Zealand-Dendermonde rabbits (gestational day 28; term 31) were randomized to three groups: normoxia-placebo (N-P), hyperoxia-placebo (H-P) and hyperoxia-caffeine (H-C). Lung function was assessed on postnatal day 5, along with airway morphometry, vascular morphometry and a score observing airway inflammation. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; Caffeine improved lung function by increasing lung volume [mean displaced volume N-P: 40.1 ± 6 ml/kg, H-P: 27.8 ± 8 ml/kg and H-C: 34.4 ± 7 ml/kg (p &lt; 0.05); total lung capacity: N-P: 1.17 ± 0.1 ml, H-P: 0.67 ± 0.1 ml and H-C: 1.1 ± 0.1 ml (p &lt; 0.05)], decreasing tissue damping [N-P: 2.7 ± 0.3 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml, H-P: 4.6 ± 0.6 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml and H-C: 3.2 ± 0.4 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml (p &lt; 0.05)], elastance [N-P: 9.3 ± 2.4 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml, H-P: 19.2 ± 7.4 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml and H-C: 10.7 ± 2 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml (p &lt; 0.05)] and compliance [N-P: 0.06 ± 0.01 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml, H-P: 0.054 ± 0.01 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml and H-C: 0.07 ± 0.013 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml (p &lt; 0.05)]. Caffeine also improved histology by decreasing alveolar size [linear intercepts; N-P: 83.6 ± 1.7, H-P: 82.9 ± 1.6 and H-C: 67.3 ± 1.4 (p &lt; 0.05)], increasing radial alveolar count (N-P: 6.6 ± 0.5, H-P: 5.7 ± 0.6 and H-C: 7.05 ± 0.5) and decreasing the acute inflammation score [N-P: 0.3 ± 0.1, H-P: 0.5 ± 0.1 and H-C: 0.4 ± 0.1 (p &lt; 0.05)]. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; In preterm rabbits, caffeine reduces the functional, architectural and inflammatory pulmonary changes induced by hyperoxia in the lung.</jats:p>caffeinebronchopulmonary dysplasiaanimal modelshyperoxic exposurelung diseaseResource Types::text::journal::journal article