JIMENEZ GREZ, JULIO ALBERTO

Preferred name
JIMENEZ GREZ, JULIO ALBERTO
Main Affiliation
Email
jjimenez@udd.cl
Scopus Author ID
56197898600

Settings

Now showing 1 - 10 of 15
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Tissuepatch is biocompatible and seals iatrogenic membrane defects in a rabbit model
    (2018)
    Chafika Mazouni
    ;
    Geraldine Porcu-Buisson
    ;
    Nadine Girard
    ;
    R. Sakr
    ;
    Dominique Figarella-Ballanger
      3Scopus© Citations 12
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Nanoparticle-induced inflammation can increase tumor malignancy
    (2018)
    Bella B. Manshian
    ;
    Jennifer Poelmans
    ;
    Shweta Saini
    ;
    Suman Pokhrel
    ;
    Julio Jiménez Grez  
      6Scopus© Citations 24
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Caffeine Prevents Hyperoxia-Induced Functional and Structural Lung Damage in Preterm Rabbits
    (2016)
    Taro Nagatomo
    ;
    Julio Jiménez  
    ;
    Jute Richter
    ;
    Siegrid De Baere
    ;
    Jeroen Vanoirbeek
    <jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Caffeine is a commonly used drug for apnea of prematurity. It may, however, also have a beneficial effect on bronchopulmonary dysplasia (BPD), which is the most common complication of extreme preterm birth. &lt;b&gt;&lt;i&gt;Objectives:&lt;/i&gt;&lt;/b&gt; To study the inflammatory, structural and functional effects of caffeine in an animal model of BPD. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Preterm New Zealand-Dendermonde rabbits (gestational day 28; term 31) were randomized to three groups: normoxia-placebo (N-P), hyperoxia-placebo (H-P) and hyperoxia-caffeine (H-C). Lung function was assessed on postnatal day 5, along with airway morphometry, vascular morphometry and a score observing airway inflammation. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; Caffeine improved lung function by increasing lung volume [mean displaced volume N-P: 40.1 ± 6 ml/kg, H-P: 27.8 ± 8 ml/kg and H-C: 34.4 ± 7 ml/kg (p &lt; 0.05); total lung capacity: N-P: 1.17 ± 0.1 ml, H-P: 0.67 ± 0.1 ml and H-C: 1.1 ± 0.1 ml (p &lt; 0.05)], decreasing tissue damping [N-P: 2.7 ± 0.3 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml, H-P: 4.6 ± 0.6 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml and H-C: 3.2 ± 0.4 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml (p &lt; 0.05)], elastance [N-P: 9.3 ± 2.4 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml, H-P: 19.2 ± 7.4 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml and H-C: 10.7 ± 2 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml (p &lt; 0.05)] and compliance [N-P: 0.06 ± 0.01 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml, H-P: 0.054 ± 0.01 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml and H-C: 0.07 ± 0.013 cm H&lt;sub&gt;2&lt;/sub&gt;O/ml (p &lt; 0.05)]. Caffeine also improved histology by decreasing alveolar size [linear intercepts; N-P: 83.6 ± 1.7, H-P: 82.9 ± 1.6 and H-C: 67.3 ± 1.4 (p &lt; 0.05)], increasing radial alveolar count (N-P: 6.6 ± 0.5, H-P: 5.7 ± 0.6 and H-C: 7.05 ± 0.5) and decreasing the acute inflammation score [N-P: 0.3 ± 0.1, H-P: 0.5 ± 0.1 and H-C: 0.4 ± 0.1 (p &lt; 0.05)]. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; In preterm rabbits, caffeine reduces the functional, architectural and inflammatory pulmonary changes induced by hyperoxia in the lung.</jats:p>
      6Scopus© Citations 50
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Scopus© Citations 7  4
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    A semi-automated method for unbiased alveolar morphometry: Validation in a bronchopulmonary dysplasia model
    (2020)
    Thomas Salaets
    ;
    Bieke Tack
    ;
    André Gie
    ;
    Benjamin Pavie
    ;
    Nikhil Sindhwani
    Scopus© Citations 23  1
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
      19Scopus© Citations 36
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Complementary Effect of Maternal Sildenafil and Fetal Tracheal Occlusion Improves Lung Development in the Rabbit Model of Congenital Diaphragmatic Hernia
    (2020)
    Francesca Maria Russo
    ;
    Marina Gabriela Monteiro Carvalho Mori Da Cunha
    ;
    Julio Jimenez  
    ;
    Flore Lesage
    ;
    Mary Patrice Eastwood
    <jats:sec> <jats:title>Objective:</jats:title> <jats:p>To evaluate the effect of combining antenatal sildenafil with fetal tracheal occlusion (TO) in fetal rabbits with surgically induced congenital diaphragmatic hernia (CDH).</jats:p> </jats:sec> <jats:sec> <jats:title>Background:</jats:title> <jats:p>Although antenatal sildenafil administration rescues vascular abnormalities in lungs of fetal rabbits with CDH, it only partially improves airway morphometry. We hypothesized that we could additionally stimulate lung growth by combining this medical treatment with fetal TO.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods:</jats:title> <jats:p>CDH was created on gestational day (GD)23 (n=54). Does were randomized to receive either sildenafil 10 mg/kg/d or placebo by subcutaneous injection from GD24 to GD30. On GD28, fetuses were randomly assigned to TO or sham neck dissection. At term (GD30) fetuses were delivered, ventilated, and finally harvested for histological and molecular analyses. Unoperated littermates served as controls.</jats:p> </jats:sec> <jats:sec> <jats:title>Results:</jats:title> <jats:p>The lung-to-body-weight ratio was significantly reduced in sham-CDH fetuses either (1.2 ± 0.3% vs 2.3 ± 0.3% in controls, <jats:italic toggle="yes">P</jats:italic>=0.0003). Sildenafil had no effect on this parameter, while CDH fetuses undergoing TO had a lung-to-body-weight ratio comparable to that of controls (2.5 ± 0.8%, <jats:italic toggle="yes">P</jats:italic>&lt;0.0001). Sildenafil alone induced an improvement in the mean terminal bronchiolar density (2.5 ± 0.8 br/mm<jats:sup>2</jats:sup> vs 3.5 ± 0.9 br/mm<jats:sup>2</jats:sup>, <jats:italic toggle="yes">P</jats:italic>=0.043) and lung mechanics (static elastance 61 ± 36 cmH<jats:sub>2</jats:sub>O /mL vs 113 ± 40 cmH<jats:sub>2</jats:sub>O/mL, <jats:italic toggle="yes">P</jats:italic>=0.008), but both effects were more pronounced in fetuses undergoing additional TO (2.1 ± 0.8 br/mm<jats:sup>2</jats:sup>, <jats:italic toggle="yes">P</jats:italic>=0.001 and 31 ± 9 cmH<jats:sub>2</jats:sub>O/mL, <jats:italic toggle="yes">P</jats:italic>&lt;0.0001 respectively). Both CDH-sham and CDH-TO fetuses treated with placebo had an increased medial wall thickness of peripheral pulmonary vessels (41.9 ± 2.9% and 41.8 ± 3.2%, vs 24.0 ± 2.9% in controls, <jats:italic toggle="yes">P</jats:italic>&lt;0.0001). CDH fetuses treated with sildenafil, either with or without TO, had a medial thickness in the normal range (29.4% ± 2.6%). Finally, TO reduced gene expression of vascular endothelial growth factor and surfactant protein A and B, but this effect was counteracted by sildenafil.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion:</jats:title> <jats:p>In the rabbit model for CDH, the combination of maternal sildenafil and TO has a complementary effect on vascular and parenchymal lung development.</jats:p> </jats:sec>
      3Scopus© Citations 19
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Preterm birth impairs postnatal lung development in the neonatal rabbit model
    (2020)
    Thomas Salaets
    ;
    Margo Aertgeerts
    ;
    André Gie
    ;
    Janne Vignero
    ;
    Derek de Winter
    <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Bronchopulmonary dysplasia continues to cause important respiratory morbidity throughout life, and new therapies are needed. The common denominator of all BPD cases is preterm birth, however most preclinical research in this area focusses on the effect of hyperoxia or mechanical ventilation. In this study we investigated if and how prematurity affects lung structure and function in neonatal rabbits.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>Pups were delivered on either day 28 or day 31. For each gestational age a group of pups was harvested immediately after birth for lung morphometry and surfactant protein B and C quantification. All other pups were hand raised and harvested on day 4 for the term pups and day 7 for the preterm pups (same corrected age) for lung morphometry, lung function testing and qPCR. A subset of pups underwent microCT and dark field imaging on day 0, 2 and 4 for terms and on day 0, 3, 5 and 7 for preterms.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Preterm pups assessed at birth depicted a more rudimentary lung structure (larger alveoli and thicker septations) and a lower expression of surfactant proteins in comparison to term pups. MicroCT and dark field imaging revealed delayed lung aeration in preterm pups, in comparison to term pups. Preterm birth led to smaller pups, with smaller lungs with a lower alveolar surface area on day 7/day 4. Furthermore, preterm birth affected lung function with increased tissue damping, tissue elastance and resistance and decreased dynamic compliance. Expression of vascular endothelial growth factor (VEGFA) was significantly decreased in preterm pups, however in the absence of structural vascular differences.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>Preterm birth affects lung structure and function at birth, but also has persistent effects on the developing lung. This supports the use of a preterm animal model, such as the preterm rabbit, for preclinical research on BPD. Future research that focuses on the identification of pathways that are involved in in-utero lung development and disrupted by pre-term birth, could lead to novel therapeutic strategies for BPD.</jats:p> </jats:sec>
    Scopus© Citations 23  1
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Modulation of the Early Host Response to Electrospun Polylactic Acid Matrices by Mesenchymal Stem Cells from the Amniotic Fluid
    (2018)
    Flore Lesage
    ;
    Sabiniano Roman
    ;
    Savitree Pranpanus
    ;
    Simona Ospitalieri
    ;
    Silvia Zia
    <jats:p> Purpose The reconstruction of congenital diaphragmatic hernia or other congenital soft tissue defects often requires implants. These can be either degradable or permanent, each having their advantages. Whatever type is being used, the host response induced by implants plays a crucial role to determine the outcome. Macrophages are pivotal during implant remodeling; they are plastic and acquire in response to environmental stimuli either an inflammatory status and mediate subsequent fibrosis or a regulatory status and facilitate functional remodeling. Matrices engineered with mesenchymal stem cells (MSCs) have the capacity to modulate the host immune reaction. MSCs are believed to promote constructive remodeling of the implant through a regulatory macrophage response among others. Herein, we evaluate this potential of MSC derived from the amniotic fluid (AF-MSC), an interesting MSC type for neonatal reconstruction, on electrospun polylactic acid (PLA) scaffolds.</jats:p><jats:p> Methods We seeded AF-MSC at a density of 1.105/cm2 on electrospun PLA matrices and determined cell viability. In vivo, we used cell-seeded or cell-free PLA matrices for subcutaneous implantation in immune competent rats. The host immune response was evaluated by histomorphometry at 14 days postoperatively.</jats:p><jats:p> Results The PLA matrix supported adherence and proliferation of AF-MSC. Fourteen days after implantation, PLA matrices were well penetrated by inflammatory cells, new blood vessels, and collagen fibers. AF-MSC–seeded scaffolds were associated with a similar response yet with a decreased number of eosinophils, increased matrix degradation and collagen fiber deposition compared with controls. The amount of total macrophages and of M2-subtype was similar for all animals.</jats:p><jats:p> Conclusion Electrospun PLA matrices are a suitable substrate for short-term culture of AF-MSC. In rats, addition of AF-MSC to PLA matrices modulates the host response after subcutaneous implantation, yet without a difference in macrophage profile compared with control.</jats:p>
      6Scopus© Citations 6
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Scopus© Citations 12  1