Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells.
Journal
Biological Research
ISSN
0717-6287
Date Issued
2019
Author(s)
Juan Palacios-Moreno
Cecilia Rubio
Wanda Quilhot
M. Fernanda Cavieres
Eduardo de la Peña
Natalia V. Quiñones
Hugo Díaz
Carlos F. Henríquez-Roldán
Caroline R. Weinstein-Oppenheimer
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:title>Abstract</jats:title>
<jats:sec>
<jats:title>Background</jats:title>
<jats:p>Epanorin (EP) is a secondary metabolite of the <jats:italic>Acarospora</jats:italic> lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP’s mutagenic activity was assessed using the <jats:italic>Salmonella typhimurium</jats:italic> reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP’s lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusion</jats:title>
<jats:p>EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Background</jats:title>
<jats:p>Epanorin (EP) is a secondary metabolite of the <jats:italic>Acarospora</jats:italic> lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP’s mutagenic activity was assessed using the <jats:italic>Salmonella typhimurium</jats:italic> reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP’s lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusion</jats:title>
<jats:p>EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.</jats:p>
</jats:sec>
Cite this document
Palacios-Moreno, J., Rubio, C., Quilhot, W., Cavieres, M. F., De La Peña, E., Quiñones, N. V., Díaz, H., Carrión, F., Henríquez-Roldán, C. F., & Weinstein-Oppenheimer, C. R. (2019). Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells. Biological Research, 52(1), 55. https://doi.org/10.1186/s40659-019-0261-4
Subjects
epanorin
;
cancer
;
cytotoxicity
;
mutagenesis
;
cell cycle
;
apoptosis
;
antineoplastic agents
;
apoptosis
;
breast neoplasms
;
cell proliferation
;
dna fragmentation
;
female
;
flow cytometry
;
humans
;
lichens
;
mcf-7 cells
;
antineoplastic agent
;
apoptosis
;
breast tumor
;
cell proliferation
;
chemistry
;
dna fragmentation
;
drug effect
;
female
;
flow cytometry
;
human
;
isolation and purification
;
lichen (organism)
;
mcf-7 cell line