Neonatal Mesenchymal Stem Cell Treatment Improves Myelination Impaired by Global Perinatal Asphyxia in Rats
Journal
International Journal of Molecular Sciences
ISSN
1422-0067
Date Issued
2021
Author(s)
Andrea Tapia-Bustos
Carolyne Lespay-Rebolledo
Valentina Vío
Ronald Pérez-Lobos
Emmanuel Casanova-Ortiz
Mario Herrera-Marschitz
Paola Morales
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:p>The effect of perinatal asphyxia (PA) on oligodendrocyte (OL), neuroinflammation, and cell viability was evaluated in telencephalon of rats at postnatal day (P)1, 7, and 14, a period characterized by a spur of neuronal networking, evaluating the effect of mesenchymal stem cell (MSCs)-treatment. The issue was investigated with a rat model of global PA, mimicking a clinical risk occurring under labor. PA was induced by immersing fetus-containing uterine horns into a water bath for 21 min (AS), using sibling-caesarean-delivered fetuses (CS) as controls. Two hours after delivery, AS and CS neonates were injected with either 5 μL of vehicle (10% plasma) or 5 × 104 MSCs into the lateral ventricle. Samples were assayed for myelin-basic protein (MBP) levels; Olig-1/Olig-2 transcriptional factors; Gglial phenotype; neuroinflammation, and delayed cell death. The main effects were observed at P7, including: (i) A decrease of MBP-immunoreactivity in external capsule, corpus callosum, cingulum, but not in fimbriae of hippocampus; (ii) an increase of Olig-1-mRNA levels; (iii) an increase of IL-6-mRNA, but not in protein levels; (iv) an increase in cell death, including OLs; and (v) MSCs treatment prevented the effect of PA on myelination, OLs number, and cell death. The present findings show that PA induces regional- and developmental-dependent changes on myelination and OLs maturation. Neonatal MSCs treatment improves survival of mature OLs and myelination in telencephalic white matter.</jats:p>
Cite this document
Tapia-Bustos, A., Lespay-Rebolledo, C., Vío, V., Pérez-Lobos, R., Casanova-Ortiz, E., Ezquer, F., Herrera-Marschitz, M., & Morales, P. (2021). Neonatal mesenchymal stem cell treatment improves myelination impaired by global perinatal asphyxia in rats. International Journal of Molecular Sciences, 22(6), 3275. https://doi.org/10.3390/ijms22063275
Project(s)
Subjects
hypomyelination
;
oligodendrocyte
;
myelination
;
neonatal asphyxia
;
ischemia
;
apoptosis
;
mesenchymal stem cells
;
telencephalon
;
rat brain
;
neuroinflammation
;
periventricular leukomalacia
;
animals
;
animals, newborn
;
apgar score
;
asphyxia
;
biomarkers
;
brain
;
cell differentiation
;
cell survival
;
cytokines
;
gene expression
;
hippocampus
;
immunohistochemistry
;
inflammation mediators
;
mesenchymal stem cell transplantation
;
mesenchymal stem cells
;
myelin sheath
;
neuroglia
;
oligodendroglia
;
rats
;
rna, messenger
;
interleukin 6
;
messenger rna
;
myelin basic protein
;
oligodendrocyte transcription factor 2
;
autacoid
;
biological marker
;
cytokine
;
messenger rna
;
animal cell
;
animal experiment
;
animal model
;
animal tissue
;
article
;
brain lateral ventricle
;
breathing rate
;
cell maturation
;
cell viability
;
cingulum (brain)
;
confocal microscopy
;
controlled study
;
corpus callosum
;
external capsule
;
female
;
fetus
;
hippocampal fimbria
;
immunoreactivity
;
mesenchymal stem cell transplantation
;
mrna expression level
;
myelinated nerve
;
myelination
;
nervous system inflammation
;
neuroprotection
;
newborn
;
nonhuman
;
oligodendroglia
;
perinatal asphyxia
;
perinatal development
;
protein expression level
;
rat
;
survival rate
;
telencephalon
;
uterus horn
;
animal
;
apgar score
;
asphyxia
;
brain
;
cell differentiation
;
cell survival
;
cytology
;
gene expression
;
genetics
;
glia
;
hippocampus
;
immunohistochemistry
;
immunology
;
mesenchymal stem cell
;
metabolism
;
myelin sheath
;
oligodendroglia
;
pathology