Streamlined computational pipeline for genetic background characterization of genetically engineered mice based on next generation sequencing data

Journal
BMC Genomics
ISSN
1471-2164
Date Issued
2019
Author(s)
C. Farkas
F. Fuentes-Villalobos
F. Benavides
A. F. Castro
R. Pincheira
Type
Resource Types::text::journal::journal article
Scopus ID
2-s2.0-85061496998
WoS ID
WOS:000458729000003
DOI
URL
Cite this document
Farkas, C., Fuentes-Villalobos, F., Rebolledo-Jaramillo, B., Benavides, F., Castro, A. F., & Pincheira, R. (2019). Streamlined computational pipeline for genetic background characterization of genetically engineered mice based on next generation sequencing data. BMC Genomics, 20(1), 131. https://doi.org/10.1186/s12864-019-5504-9
Subjects
sequencing

; 

congenic mouse

; 

knockout mouse

; 

genomic variation

; 

genetic interactions

; 

modifier genes

; 

genetic background

; 

rna-seq variant calling

; 

qpcr validation

; 

ang

; 

cdkn1a

; 

sall2

; 

animals

; 

animals, genetically modified

; 

computational biology

; 

cyclin-dependent kinase inhibitor p21

; 

embryonic stem cells

; 

gene expression regulation

; 

genes, modifier

; 

genetic background

; 

high-throughput nucleotide sequencing

; 

intracellular signaling peptides and proteins

; 

mice

; 

mice, 129 strain

; 

mice, congenic

; 

mice, inbred c57bl

; 

mice, knockout

; 

polymorphism, single nucleotide

; 

quantitative trait loci

; 

sequence analysis, rna

; 

whole exome sequencing

; 

cdkn1a protein, mouse

; 

cyclin dependent kinase inhibitor 1a

; 

sall2 protein, mouse

; 

signal peptide

; 

article

; 

female

; 

gene expression

; 

gene identification

; 

gene locus

; 

genetic drift

; 

genetic engineering

; 

genotyping technique

; 

introgression

; 

modifier gene

; 

mouse

; 

next generation sequencing

; 

nonhuman

; 

rna sequence

; 

sequence analysis

; 

whole exome sequencing

; 

whole genome sequencing

; 

129 mouse

; 

animal

; 

biology

; 

c57bl mouse

; 

congenic strain

; 

embryonic stem cell

; 

gene expression regulation

; 

genetic background

; 

genetics

; 

high throughput sequencing

; 

knockout mouse

; 

physiology

; 

procedures

; 

quantitative trait locus

; 

single nucleotide polymorphism

; 

transgenic animal