Cells from discarded dressings differentiate chronic from acute wounds in patients with Epidermolysis Bullosa
Journal
Scientific Reports
ISSN
2045-2322
Date Issued
2020
Author(s)
Christina Guttmann-Gruber
Birgit Tockner
Anja Diem
Alfred Klausegger
Glenda Cofré-Araneda
Olga Figuera
Yessia Hidalgo
Pilar Morandé
Raymond J. Cho
Heather I. Rishel
M. Peter Marinkovich
Joyce M. C. Teng
Timothy G. Webster
Marco Prisco
Luis H. Eraso
Josefina Piñon Hofbauer
Andrew P. South
Type
Resource Types::text::journal::journal article
URL Institutional Repository
Abstract
<jats:title>Abstract</jats:title><jats:p>Impaired wound healing complicates a wide range of diseases and represents a major cost to healthcare systems. Here we describe the use of discarded wound dressings as a novel, cost effective, accessible, and non-invasive method of isolating viable human cells present at the site of skin wounds. By analyzing 133 discarded wound dressings from 51 patients with the inherited skin-blistering disease epidermolysis bullosa (EB), we show that large numbers of cells, often in excess of 100 million per day, continually infiltrate wound dressings. We show, that the method is able to differentiate chronic from acute wounds, identifying significant increases in granulocytes in chronic wounds, and we show that patients with the junctional form of EB have significantly more cells infiltrating their wounds compared with patients with recessive dystrophic EB. Finally, we identify subsets of granulocytes and T lymphocytes present in all wounds paving the way for single cell profiling of innate and adaptive immune cells with relevance to wound pathologies. In summary, our study delineates findings in EB that have potential relevance for all chronic wounds, and presents a method of cellular isolation that has wide reaching clinical application.</jats:p>
Cite this document
Fuentes, I., Guttmann-Gruber, C., Tockner, B., Diem, A., Klausegger, A., Cofré-Araneda, G., Figuera, O., Hidalgo, Y., Morandé, P., Palisson, F., Rebolledo-Jaramillo, B., Yubero, M. J., Cho, R. J., Rishel, H. I., Marinkovich, M. P., Teng, J. M. C., Webster, T. G., Prisco, M., Eraso, L. H., … South, A. P. (2020). Cells from discarded dressings differentiate chronic from acute wounds in patients with Epidermolysis Bullosa. Scientific Reports, 10(1), 15064. https://doi.org/10.1038/s41598-020-71794-1
Subjects
acute disease
;
adult
;
bandages
;
cell separation
;
chronic disease
;
epidermolysis bullosa
;
granulocytes
;
humans
;
male
;
t-lymphocytes
;
wound healing
;
acute disease
;
adult
;
bandage
;
cell separation
;
chronic disease
;
epidermolysis bullosa
;
granulocyte
;
human
;
male
;
metabolism
;
pathology
;
t lymphocyte
;
wound healing