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Coagulation Factor Xa Promotes Solid Tumor Growth, Experimental Metastasis and Endothelial Cell Activation

2019 , Maximiliano Arce , Mauricio P. Pinto , Macarena Galleguillos , Catalina Muñoz , Soledad Lange , Carolina Ramirez , Rafaela Erices , Pamela Gonzalez , Ethel Velasquez , Fabián Tempio , Mercedes N. Lopez , Flavio Salazar-Onfray , Kelly Cautivo , Alexis M. Kalergis , Sebastián Cruz , Álvaro Lladser , LOBOS GONZALEZ, LORENA DE LOURDES , Guillermo Valenzuela , Nixa Olivares , Claudia Sáez , Tania Koning , Fabiola A. Sánchez , Patricia Fuenzalida , Alejandro Godoy , Pamela Contreras Orellana , Lisette Leyton , Roberta Lugano , Anna Dimberg , Andrew F.G. Quest , Gareth I. Owen

Hypercoagulable state is linked to cancer progression; however, the precise role of the coagulation cascade is poorly described. Herein, we examined the contribution of a hypercoagulative state through the administration of intravenous Coagulation Factor Xa (FXa), on the growth of solid human tumors and the experimental metastasis of the B16F10 melanoma in mouse models. FXa increased solid tumor volume and lung, liver, kidney and lymph node metastasis of tail-vein injected B16F10 cells. Concentrating on the metastasis model, upon coadministration of the anticoagulant Dalteparin, lung metastasis was significantly reduced, and no metastasis was observed in other organs. FXa did not directly alter proliferation, migration or invasion of cancer cells in vitro. Alternatively, FXa upon endothelial cells promoted cytoskeleton contraction, disrupted membrane VE-Cadherin pattern, heightened endothelial-hyperpermeability, increased inflammatory adhesion molecules and enhanced B16F10 adhesion under flow conditions. Microarray analysis of endothelial cells treated with FXa demonstrated elevated expression of inflammatory transcripts. Accordingly, FXa treatment increased immune cell infiltration in mouse lungs, an effect reduced by dalteparin. Taken together, our results suggest that FXa increases B16F10 metastasis via endothelial cell activation and enhanced cancer cell-endothelium adhesion advocating that the coagulation system is not merely a bystander in the process of cancer metastasis.

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Inactivated Vaccine-Induced SARS-CoV-2 Variant-Specific Immunity in Children

2022 , Jorge A. Soto , Felipe Melo-González , Cristián Gutierrez-Vera , Bárbara M. Schultz , Roslye V. Berríos-Rojas , Daniela Rivera-Pérez , Alejandro Piña-Iturbe , Guillermo Hoppe-Elsholz , Luisa F. Duarte , Yaneisi Vázquez , Daniela Moreno-Tapia , Mariana Ríos , Pablo A. Palacios , Richard Garcia-Betancourt , Álvaro Santibañez , Gaspar A. Pacheco , Constanza Mendez , Catalina A. Andrade , Pedro H. Silva , Benjamín Diethelm-Varela , Patricio Astudillo , Mario Calvo , Antonio Cárdenas , Marcela González , Macarena Goldsack , Valentina Gutiérrez , Marcela Potin , SCHILLING REDLICH, ANDREA INGRID , Lorena I. Tapia , Loreto Twele , Rodolfo Villena , Alba Grifoni , Alessandro Sette , Daniela Weiskopf , Rodrigo A. Fasce , Jorge Fernández , Judith Mora , Eugenio Ramírez , Aracelly Gaete-Argel , Mónica L. Acevedo , Fernando Valiente-Echeverría , Ricardo Soto-Rifo , Angello Retamal-Díaz , Nathalia Muñoz-Jofré , Xing Meng , Qianqian Xin , Eduardo Alarcón-Bustamante , José V. González-Aramundiz , Nicole Le Corre , María Javiera Álvarez-Figueroa , CEA GONZALEZ, PABLO ANTONIO , Katia Abarca , Cecilia Perret , Leandro J. Carreño , Susan M. Bueno , Alexis M. Kalergis , Patricio Astudillo Paredes , Epifanía Hernández Jara , Héctor Morán Fernández , Javiera Arenas Urra , Stephani Ascui Baeza , María Olivia Cabrera , José Romero Muñoz , Gonzalo Alarcón Andrade , Rocío Rodríguez Espósito , Anwar Gutiérrez Silva , Fernanda Pérez Gutiérrez , Alma Muñoz Muñoz , Marcela Potin Santander , Sofia López , Tania Weil , Macarena Goldsack , Deidyland Arenas , Javiera Moore , ARAYA CASTRO, PAULINA ANDREA , Lorena Pilicita , Vania Valenzuela , Catalina Campos , Mauricio Soto , SCHILLING REDLICH, ANDREA INGRID , Alberto Trautmann , Ana Fritis , Daniela Pavez , Javiera Arancibia , Lilian Rubio , Paula Viviani , Vinka Basic , Cassandra Cárcamo , Mario Calvo Gil , Marisol Wenzel , Nicole Carey , Roberto Burgos , Loreto Twele , Daniel Beltrán , Silvana Grandón , Carlos Tovar , Marcela González , Daniela Fuentes , Teresa Ramírez , Mariela Cepeda Corrales , Nataly Martínez López , Valentina Gutiérrez , Felipe Reyes , Armando Lavayen , Melissa González , Monserrat Gutiérrez , Noris Rengifo , Carla Ortega , Florencia Saver , Lorena Tapia , Mirta Acuña , Javiera Albornoz , Tania Cariqueo , Alejandro Velásquez , Yennybeth Leiva Chamorro , Rodolfo Villena , Paola Flores , Francisca Bartsch , Belén Sepúlveda , Daniela Garmendia , Antonio Cárdenas , Angello Retamal , Carmen Ludeña , Lorena González , Carolina Hermosilla , Gustavo Keilhold , Francisco Cammarata-Scalisi , Jessica Álvarez , Jessica Romero , Pía Villarroel , Francisca Muñoz , Jorge Maya , Andrés Canales , Margarita K. Lay , Christian Muñoz , Roylester Araya , Kanta Subbarao

This work evaluated the immune response induced by two doses of CoronaVac separated by 4 weeks in healthy children and adolescents in Chile. To date, few studies have described the effects of CoronaVac in the pediatric population.