Abstract Introduction Ambulatory blood pressure (BP) monitoring encapsulates 24-h BP patterns across the sleep-wake cycle and provides a comprehensive assessment of BP regulation with certain features, such as sleep-time dipping, that can better predict health outcomes than traditional office BP measurements. However, 24-h BP patterns remain understudied in Latino populations, especially older adults. This pilot study investigated the feasibility of continuously ambulatory BP monitoring blood pressure with portable devices in Latinos with dementia. Methods Eight participants (4 with Alzheimer’s disease, 1 with Frontotemporal dementia, 3 controls; age range 45.8-80.3 years) from the ReDLat cohort (Multi-Partner Consortium to Expand Dementia Research in Latin America) were enrolled for a 48-h ambulatory BP assessment with hourly measurements using a portable arm-cuff device. Participants also answered six questions to evaluate: (1) device adaption, (2) measurement frequency tolerability, (3) study duration acceptability, (4) perceived value of the assessment, (5) willingness to perform the assessment again, and (6) likelihood of recommending the assessment to others. Results The study yielded high-quality recordings with minimal missing BP measurements (range: 0%-14.1%; mean: 8.9%). Device adaptation improved over time, with 75% (6/8) reporting better tolerance on the second day and 25% (2/8) reporting consistently good tolerance across both days. All participants reported that hourly BP measurement was tolerable, and majority (7/8, 87.5%) found 48-h monitoring acceptable. Seven participants (87.5%) believed that the assessment was valuable, were willing to participate again, and likely recommend to others the assessment. Conclusion Our findings demonstrate that 48-hour ambulatory BP monitoring is feasible and well-tolerated among older Latino adults with mild to moderate dementia in Latin American settings. Further feasibility testing should be performed in large samples and other populations, including individuals at later stages of dementia and in different countries.

Background: TDetection of distal radioulnar joint (DRUJ) instability has proven inconsistent despite numerous examination maneuvers. Computed tomography (CT) has been suggested as a modality for evaluating DRUJ instability; however, without stress across the DRUJ, it fails to reliably identify this. No study has simultaneously assessed stress CT with clinical stress maneuvers. As such, the purpose of this study was to compare both methods in stable and unstable wrists. Methods: An arthrometer was developed to evaluate a clinical stress test of the DRUJ in various degrees of forearm rotation. In each forearm position, specimens were subjected to standardized volar and dorsal loads to simulate clinical stress to the DRUJ. Computed tomography images were acquired in each position with additional unstressed images. The triangular fibrocartilage complex (TFCC) was then sectioned to simulate DRUJ instability. Results: Nine upper extremities were used. The arthrometer could detect a significant difference between sectioned and intact TFCCs in the supinated forearm position. A large proportion of the translation measured by the arthrometer was due to rotation within the clamps. In the unstressed state, CT analysis using the radioulnar ratio failed to show significant differences in DRUJ stability for any forearm orientation. By applying stress across the DRUJ, CT analysis of ulnar translation along the sigmoid notch showed significant differences between TFCC intact and sectioned wrists. Conclusions: Clinical examination maneuvers and arthrometers are observing a rotational component that occurs due to the inability to directly clamp the underlying bone, whereas stress CT is more accurate for quantifying underlying bony translation. Unstressed CT analysis did not identify instability.

Dysregulated A>I(G) RNA editing, which is mainly catalyzed by ADAR1 and is a type of post-transcriptional modification, has been linked to cancer. A low response to therapy in breast cancer (BC) is a significant contributor to mortality. However, it remains unclear if there is an association between A>I(G) RNA-edited sites and sensitivity to genotoxic drugs. To address this issue, we employed a stringent bioinformatics approach to identify differentially RNA-edited sites (DESs) associated with low or high sensitivity (FDR 0.1, log2 fold change 2.5) according to the IC50 of PARP inhibitors, anthracyclines, and alkylating agents using WGS/RNA-seq data in BC cell lines. We then validated these findings in patients with basal subtype BC. These DESs are mainly located in non-coding regions, but a lesser proportion in coding regions showed predicted deleterious consequences. Notably, some of these DESs are previously reported as oncogenic variants, and in genes related to DNA damage repair, drug metabolism, gene regulation, the cell cycle, and immune response. In patients with BC, we uncovered DESs predominantly in immune response genes, and a subset with a significant association (log-rank test p < 0.05) between RNA editing level in LSR, SMPDL3B, HTRA4, and LL22NC03-80A10.6 genes, and progression-free survival. Our findings provide a landscape of RNA-edited sites that may be involved in drug response mechanisms, highlighting the value of A>I(G) RNA editing in clinical outcomes for BC.